文献类型：期刊文献

中文题名：当归挥发油通过抑制PI3K/Akt/mTOR信号转导通路影响人结肠癌LOVO细胞自噬研究

英文题名：Study on Volatile Oil of Angelicae Sinensis Radix Affects Autophagy in Human Colorectal Cancer LOVO Cells by Inhibiting PI3K/Akt/mTOR Signal Transduction Pathway

作者：朱丽娟[1,2];罗建云[3];宋润泽[4];王丽娟[1,2];张安平[4];臧凯宏[1,2]

第一作者：朱丽娟

机构：[1]甘肃中医药大学药学院,兰州730000;[2]甘肃省中药药理与毒理学重点实验室,兰州730000;[3]陕西省西安市医疗保障局,西安710021;[4]兰州大学第二医院血管外科,兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2022

卷号：39

期号：4

起止页码：437

中文期刊名：中国现代应用药学

外文期刊名：Chinese Journal of Modern Applied Pharmacy

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：甘肃省高等学校科研基金资助项目(2018-A050);甘肃省中药药理与毒理学重点实验室开放基金资助项目(ZDSYS-KJ-2018-008)

语种：中文

中文关键词：当归挥发油;LOVO细胞;自噬;PI3K/Akt/mTOR信号转导通路

外文关键词：volatile oil of Angelicae Sinensis Radix;LOVO cells;autophagy;PI3K/Akt/mTOR pathway

摘要：目的研究当归挥发油(volatile oil of Angelicae Sinensis Radix,VOAS)对人结直肠癌LOVO细胞自噬的影响及其分子机制。方法用不同浓度的VOAS(6.25,12.5,25,50和100μg·mL^(-1))作用于人结肠癌LOVO细胞48 h,CCK8比色法检测VOAS对LOVO细胞增殖的抑制作用。将VOAS 50μg·mL^(-1)作用于LOVO细胞,AO荧光染色和透射电镜观察细胞自噬情况;Western blotting观察VOAS对自噬相关蛋白LC3B、Beclin-1、Atg5及PI3K/Akt/mTOR信号转导通路的影响。结果6.25~100μg·mL^(-1)的VOAS能够抑制LOVO细胞的增殖,具有浓度依赖性;50μg·mL^(-1)的VOAS能够促进LOVO细胞的自噬,上调自噬相关蛋白LC3B-Ⅱ、Beclin-1及Atg5的表达,同时下调PI3K、p-Akt、p-mTOR蛋白的表达。结论VOAS能够促进人结肠癌LOVO细胞自噬,其机制可能与抑制PI3K/Akt/mTOR信号转导通路有关。
OBJECTIVE To study the effect and mechanism of volatile oil of Angelicae Sinensis Radix(VOAS)on autophagy in human colorectal cancer LOVO cells.METHODS Human colorectal cancer LOVO cells were treated with various concentrations of VOAS(6.25,12.5,25,50 and 100μg·mL^(-1))for 48 h,CCK8 assay was used to detect the effect of VOAS on the proliferation inhibition effect of cells.When treated with VOAS(50μg·mL^(-1)),the effect of VOAS on autophagy was observed by acridine orange staining and electron microscopy,Western blotting was used to detect the expression of autophagy hallmark protein(LC3B,Beclin-1,Atg5)and PI3K/Akt/mTOR signal pathway.RESULTS The 6.25-100μg·mL^(-1)VOAS could significantly inhibit the proliferation of LOVO cells in dose-dependent manner,and VOAS(50μg·mL^(-1))could promote autophagy.Furthermore,VOAS(50μg·mL^(-1))could up-regulate the expression of LC3B-Ⅱ,Beclin-1 and Atg5 protein that related to autophagy,and down-regulate the expression of PI3K,p-Akt and p-mTOR protein.CONCLUSION VOAS may induce autophagy in human colorectal cancer LOVO cells through inhibiting PI3K/Akt/mTOR signal transduction pathway.