文献类型：期刊文献

中文题名：基于多成分含量测定的红芪搓条与未搓条样品比较研究

英文题名：Comparative study on rubbed and unrubbed Hedysari Radix based on multi-component determination

作者：罗旭东[1];李昕蓉[1];李成义[1];齐鹏[1];梁婷婷[1];冯晓莉[1,2];贾妙婷[1];王燕[1]

第一作者：罗旭东

机构：[1]甘肃中医药大学;[2]敦煌医学与转化教育部重点实验室,兰州730000

第一机构：甘肃中医药大学

年份：2024

卷号：36

期号：7

起止页码：1215

中文期刊名：天然产物研究与开发

外文期刊名：Natural Product Research and Development

收录：CSTPCD;;北大核心:【北大核心2023】；CSCD:【CSCD2023_2024】；

基金：国家自然科学基金地区基金(82160730);甘肃省重点研发计划(21YF5FA133);“双一流”科研重点项目(GSSYLXM-05);敦煌医学与转化教育部重点实验室项目(DHYX20-12)。

语种：中文

中文关键词：红芪;搓条;未搓条;指纹图谱;化学计量学;芒柄花素

外文关键词：Hedysari Radix;rubbed;unrubbed;fingerprint;chemometrics;formononetin

摘要：建立搓条和未搓条红芪样品HPLC指纹图谱,结合多成分定量,比较搓条和未搓条样品的差异。采用Angilent C 18色谱柱(4.6 mm×250 mm,5μm),流动相为0.1%磷酸水溶液-乙腈(梯度洗脱),流速1.0 mL/min,柱温30℃,建立14批搓条和未搓条样品指纹图谱,通过相似度评价和化学计量学分析,研究不同红芪样品的差异性,测定毛蕊异黄酮、芒柄花素、总多糖、总黄酮等成分的含量。结果显示,搓条和未搓条样品均标定25个共有峰,指认了其中7个成分,分别为腺苷、香草酸、毛蕊异黄酮苷、芒柄花苷、毛蕊异黄酮、芒柄花素、美迪紫檀素。聚类分析、主成分分析均能很好地区分搓条和未搓条样品,正交偏最小二乘-判别分析共找到12个差异性标志物,差异显著性排序分别为峰25>峰23>峰14>芒柄花素>毛蕊异黄酮苷>毛蕊异黄酮>峰18>峰19>峰15>芒柄花苷>峰4>香草酸。含量测定结果显示,红芪搓条样品中毛蕊异黄酮、芒柄花素、浸出物、总多糖含量明显高于未搓条样品,毛蕊异黄酮苷、芒柄花苷、香草酸、总黄酮含量明显低于未搓条样品。该研究为红芪质量和搓条加工提供数据参考。
To establish the HPLC fingerprints of rubbed and unrubbed Hedysari Radix samples,the differences between rubbed and unrubbed samples were compared with multi-component quantification.The chromatographic column was Angilent C 18(4.6 mm×250 mm,5μm).The flow rate was 1.0 mL/min,and the mobile phase consisted of 0.1% phosphoric acid solution-acetonitrile(gradient elution).The column temperature was 30℃.The fingerprints of 14 batches of rubbed and unrubbed samples were established,through similarity evaluation and chemometric analysis,and the difference between rubbed and unrubbed samples was studied subsequently.The contents of components such as calycosin,formononetin,total polysaccharides,and total flavonoids were determined.The results showed that there were 25 common peaks in the fingerprint of rubbed and unrubbed samples.Seven components were identified as adenosine,vanillic acid,calycosin-7-glucoside,ononin,calycosin,formononetin,medicarpin.Both hierarchical clustering analysis and principal component analysis can be distinguished between rubbed and unrubbed samples.Orthogonal partial least squares-discriminant analysis found twelve components of difference,and the order of significance of difference was peak 25>peak 23>peak 14>formononetin>calycosin-7-glucoside>calycosin>peak 18>peak 19>peak 15>ononin>peak 4>vanillic acid.The results of content determination showed that the content of calycosin,formononetin,total polysaccharide,and extract in rubbed samples was significantly higher than in the unrubbed ones.In contrast,the contents of calycosin-7-glucoside,ononin,vanillic acid,and total flavonoids in rubbed samples were significantly lower than in the unrubbed ones.The study can provide data reference for quality and rubbing processing of Hedysari Radix.