文献类型：期刊文献

英文题名：A vaccine targeting basic fibroblast growth factor elicits a protective immune response against murine melanoma

作者：Zhang, Xiaoping[1];Li, Neng-Lian[1];Guo, Chao[1];Li, Ying-Dong[1];Luo, Lu-Lu[2];Liu, Yong-Qi[1];Duan, Yun-Yan[3];Li, Zhen-Dong[4];Xie, Xiao-Rong[1];Song, Hai-Xia[5];Yang, Li-Ping[6];An, Fang-Yu[1]

第一作者：张小平

通信作者：Li, YD[1];Li, YD[2]

机构：[1]Gansu Univ Chinese Med, Inst Integrated Tradit Chinese & Westen Med, Lanzhou, Gansu, Peoples R China;[2]Gansu Univ Chinese Med, Affiliated Hosp, Lanzhou, Gansu, Peoples R China;[3]Gansu Univ Chinese Med, Expt Teaching Ctr, Lanzhou, Gansu, Peoples R China;[4]Lanzhou Univ, Dept Ultrasound, Hosp 2, Lanzhou, Gansu, Peoples R China;[5]Tumor Hosp Gansu Prov, Dept Radiotherapy, Lanzhou, Gansu, Peoples R China;[6]Lanzhou Univ, Dept Oncol, Hosp 1, Lanzhou, Gansu, Peoples R China

第一机构：甘肃中医药大学中西医结合学院

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Inst Integrated Tradit Chinese & Westen Med, Lanzhou, Gansu, Peoples R China;[2]corresponding author), Gansu Univ Chinese Med, Dingxi East Rd 35, Lanzhou 730000, Gansu, Peoples R China.|[10735]甘肃中医药大学;[10735ed249c6606940a33]甘肃中医药大学中西医结合学院;

年份：2018

卷号：19

期号：6

起止页码：518

外文期刊名：CANCER BIOLOGY & THERAPY

收录：;Scopus(收录号：2-s2.0-85042921785);WOS:【SCI-EXPANDED(收录号:WOS:000430935100010)】；

基金：This work was supported by National Natural Science Foundation of China (NSFC) [Grant No. 81360345], Natural Science Foundation of Gansu Science and Technology Department [Grant No. 1208RJZA221], and Opening foundation of Dunhuang medicine and transformation key laboratory of ministry of education [Grant No. DHYX17-07].

语种：英文

外文关键词：Basic fibroblast growth factor; melanoma; immunotherapy

摘要：Tumor growth and metastasis are closely related to angiogenesis. Basic fibroblast growth factor( bFGF) is an angiogenic factor, and up-regulated expression of bFGF plays a crucial role in the development and metastasis of melanoma. Therefore, in this study, we sought to achieve antitumor activity by immunity targeting bFGF which would inhibit tumor angiogenesis and simultaneously induce bFGF specific cytotoxic T lymphocytes to kill melanoma cells. A human bFGF protein was used as exogenous antigen, coupled with a saponin-liposome adjuvant formulation to enhance CTL response. The results showed that the immunity induced strong immune response and produced prominent anti-cancer activities. CD31 immunohistochemistry and alginate-encapsulated tumor cell assay displayed that tumor angiogenesis was effectively inhibited. Further, the higher production of IFN-gamma and cytotoxic T lymphocyte killing assay suggested that the anti-cancer activities may mainly depend on cellular immune response, which could cause the inhibition of tumor angiogenesis and specific killing of tumor cells by bFGF-specific cytotoxic T lymphocytes. We concluded that immunotherapy targeting bFGF may be a prominent strategy for melanoma, and that the adjuvant formulation of saponin-liposome is very desirable in enhancing cytotoxic T lymphocytes response.