文献类型：期刊文献

英文题名：Synergistic Apoptotic Effect of D-Fraction From Grifola frondosa and Vitamin C on Hepatocellular Carcinoma SMMC-7721 Cells

作者：Zhao, Fei[1,2,3];Wang, Yong-Feng[4];Song, Lei[2];Jin, Jia-Xin[5];Zhang, Ya-Qing[2];Gan, Hong-Yun[2];Yang, Ke-Hu[1,3]

第一作者：Zhao, Fei

通信作者：Yang, KH[1]

机构：[1]Lanzhou Univ, Sch Basic Med Sci, Inst Integrated Tradit Chinese & Western Med, Evidence Based Med Ctr, Lanzhou, Peoples R China;[2]Nortwest Univ Nationalities, Sch Med, Lanzhou, Peoples R China;[3]Lanzhou Univ, Key Lab Evidence Based Med & Knowledge Translat G, Lanzhou, Peoples R China;[4]Gansu Univ Tradit Chinese Med, Sch Basic Med Sci, Lanzhou, Peoples R China;[5]Lanzhou Univ, Hosp 2, Clin Med Coll 2, Lanzhou, Peoples R China

第一机构：Lanzhou Univ, Sch Basic Med Sci, Inst Integrated Tradit Chinese & Western Med, Evidence Based Med Ctr, Lanzhou, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Sch Basic Med Sci, Evidence Based Med Ctr, 199 Donggang West Rd, Lanzhou 730000, Gansu, Peoples R China.

年份：2017

卷号：16

期号：2

起止页码：205

外文期刊名：INTEGRATIVE CANCER THERAPIES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000401553100008)】；

基金：The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This study was supported by the Fundamental Research Funds for the Central Universities from Northwest University for Nationalities, China (No. 1920140070) and Natural Science Foundation of Gansu Province of China (No. 145RJZA221).

语种：英文

外文关键词：Grifola frondosa; D-fraction; vitamin C; apoptosis; hepatic carcinoma; SMMC-7721

摘要：The aim of this study was to investigate the anticancer effect of a combination of D-fraction polysaccharide from Grifola frondosa (DFP) and vitamin C (VC) on hepatocellular carcinoma in vitro. DFP is a bioactive extract from the maitake mushroom. Anticancer activity was demonstrated using various concentrations of DFP alone or in combination with VC against the human hepatocarcinoma SMMC-7721 cell line. To investigate the anticancer mechanism, studies designed to detect cell apoptosis were conducted. Results from the MTT assay indicated that a combination of DFP (0.2 mg/mL) and VC (0.3 mmol/L) led to a 70% reduction in cell viability. Flow cytometry results indicated that DFP/VC treatment induced apoptosis in approximately 65% SMMC-7721 cells. Cell cycle analysis identified cell cycle arrest at the G2/M phase following DFP/VC treatment for 48 hours. In addition, cellular morphological changes were observed using transmission electron microscopy. Western blot analysis revealed that the upregulation of BAX, downregulation of Bcl-2, activation of poly-(ADP-ribose)-polymerase (PARP), and the release of cytochrome c were observed in cells treated with the combination of DFP/VC, which showed that the mechanism of anticancer activity in the SMMC-7721 hepatocarcinoma cells involved induction of apoptosis.