文献类型：期刊文献

中文题名：脑类器官在创伤性脑损伤研究中的应用

英文题名：Application of brain organoids in the study of traumatic brain injury

作者：刘可[1,2];郭文文[1,2];王颖花[2,3];张延英[1];汪永锋[1];赵亚[2];师长宏[1,2]

第一作者：刘可

机构：[1]甘肃中医药大学第一临床医学院,兰州730000;[2]空军军医大学实验动物中心,西安710032;[3]延安大学医学院,陕西延安716000

第一机构：甘肃中医药大学临床医学院

年份：2023

卷号：31

期号：5

起止页码：690

中文期刊名：中国实验动物学报

外文期刊名：Acta Laboratorium Animalis Scientia Sinica

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD_E2023_2024】；

基金：陕西省创新能力支持计划(2021PT-037);军队实验动物专项课题(SYDW2018-01)。

语种：中文

中文关键词：脑类器官;创伤性脑损伤;移植

外文关键词：brain organoids;traumatic brain injury;transplantation

摘要：创伤性脑损伤(traumatic brain injury,TBI)是由外力引起的脑正常功能破坏和/或脑组织病理性损伤。由于发生功能神经元不可逆的丧失和神经组织损伤,中枢神经系统在创伤后很难修复和再生,造成TBI患者预后存在严重后遗症。由于缺乏概括人脑特征的TBI模型,虽然有大量成功的临床前研究用于TBI治疗,但少有转化应用。脑类器官作为一种自我组装的3D组织,具有来自干细胞和器官特异性细胞类型的集合,能够在一定程度上模拟天然脑器官的结构和功能,应用于TBI研究,能够有效地解决人中枢神经系统组织获取的局限性以及人和动物之间的生物学特征不匹配问题。因此,本文通过对脑类器官的产生、特性及其在TBI模型中的应用进行综述,重点讨论了基于人多能干细胞(hPSCs)的脑类器官模拟体外TBI模型和嵌合动物TBI模型研究进展,以期为脑类器官应用于TBI损伤的研究及治疗提供新思路。
Traumatic brain injury(TBI)is the destruction of normal brain functions and/or pathological injury of brain tissue caused by external force.Because of the irreversible loss of functional neurons and nerve tissue damage,the central nervous system has difficulty repairing and regenerating after trauma,and the prognosis of TBI patients has serious sequelae.The existing TBI models cannot represent the characteristics of the human brain.Although many preclinical studies of TBI therapy have been successful,few have led to clinical translation.As a self-assembled 3D tissue with a collection of stem cells and organ-specific cell types,brain organoids simulate the structure and function of the natural brain to a certain extent.They effectively resolve the limitations of tissue acquisition in the human central nervous system and the mismatch of biological characteristics between humans and animals in TBI research.Therefore,in this review,we summarize the generation,characteristics,and application of brain organoids as TBI models and focus on the research progress of brain organoids simulated in vitro and chimeric animal models with TBI based on human pluripotent stem cells to provide new ideas for the application of brain organoids in the research and treatment of TBI injuries.