文献类型：期刊文献

中文题名：乳鼠心肌细胞原代培养方法的改进

英文题名：The improved primary cultural method of the neonatal rat cardiac myocytes

作者：王宁[1];李应东[2]

第一作者：王宁

机构：[1]甘肃中医学院医疗系,兰州市730000;[2]甘肃中医学院科研试验中心,兰州市730000

第一机构：甘肃中医药大学中医临床学院

年份：2007

卷号：5

期号：12

起止页码：920

中文期刊名：中国心血管病研究

外文期刊名：Chinese Journal of Cardiovascular Research

收录：CSTPCD

语种：中文

中文关键词：心肌,细胞;细胞培养;乳鼠

外文关键词：Myocardium, cells ; Cell culture ; Neonatal rat

摘要：目的对乳鼠的心肌细胞进行原代培养方法的改进。方法参照乳鼠心肌细胞原代培养方法,并进行改进,选用混合消化酶(0.08%胰蛋白酶+0.04%～0.06%Ⅱ型胶原酶等量加入的混合液)及DF培养液进行消化和培养,减少了对心肌细胞的损伤,差速贴壁分离纯化后,加入5-溴-α-脱氧尿嘧啶抑制成纤维细胞分裂增殖。结果本方法培养的心肌细胞存活率为95.7%,培养2～5d,视野中绝大多数为已搏动的心肌细胞和心肌细胞团,成纤维细胞无明显增殖。结论本方法是一种较为理想的乳鼠心肌细胞原代培养方法。
Objective To iemprove the method of neonatal rat primary cardiac myocytes culture. Methods Reference the method of neonatal rat primary cardiac myocytes culture and improve it, choose the mixture digestive enzymes （0.08% pancreatic enzyme and 0.04%-0.06% collagenase Ⅱ ） and DF culture medium to digest and culture cardiac myocytes, decrease the injury to cardiac myocytes, after using differential adhesion to separate and purify cardiac myocytes,add 5-Brdu to inhibit the proliferation of fibroblasts. Results The survival rate of cardiac myocytes through this method was 95.7%, culturing two to five days and observing,the majority in visual fields were pulsative cardiac myocytes and cardiac myocyte cluster, fibrobiasts proliferated unobviousiy.Conclusion This method is an ideal one to primary cardiac myocytes culture.