文献类型：期刊文献

中文题名：腰腿痛胶囊对髓核移植型腰椎间盘突出症大鼠模型背根神经节中P物质和降钙素基因相关肽含量的影响

英文题名：Effect of capsules for lumbago-leg pain on the contents of substance P and calcitonin gene-related peptide in dorsal root ganglia of rats models with lumbar disc herniation in nucleus pulposus transplantation type

作者：姜劲挺[1];安文博[2]

第一作者：姜劲挺

机构：[1]甘肃中医学院附属医院,甘肃兰州730020;[2]甘肃中医学院

第一机构：甘肃中医药大学第二附属医院

年份：2012

卷号：24

期号：6

起止页码：18

中文期刊名：中医正骨

外文期刊名：The Journal of Traditional Chinese Orthopedics and Traumatology

基金：兰州市科技计划项目(2004-1-62)

语种：中文

中文关键词：腰腿痛;椎间盘移位;腰椎;神经节,脊;P物质;降钙素基因相关肽;动物实验

外文关键词：Lumbago-leg pain ; Intervertebral disk displacement ; Lumbar vertebrae ; Ganglia, spinal ; Substance P ; Calcitonin gene-related peptide ; Animal experimentation

摘要：目的:观察腰腿痛胶囊对髓核移植型腰椎间盘突出症大鼠模型背根神经节中P物质和降钙素基因相关肽含量的影响,探讨腰腿痛胶囊治疗腰椎间盘突出症的作用机理。方法:将48只成年SD大鼠随机分为空白对照组、模型组、中药对照组及腰腿痛胶囊组,每组12只。采用髓核移植的造模方法将除空白对照组外的其余各组大鼠制成腰椎间盘突出症大鼠模型。自造模后第2天开始,腰腿痛胶囊组每日灌服腰腿痛胶囊与生理盐水配置的悬浊液,中药对照组每天灌服腰腿痛丸与生理盐水配置的悬浊液,空白对照组和模型组灌服等体积的生理盐水。连续灌胃2周后,取各组大鼠尾段髓核移植于L4～L6邻近支脊神经背根神经节,采用免疫组化法测定各组大鼠背根神经节中P物质和降钙素基因相关肽的含量。结果:①P物质平均灰度。4组大鼠P物质平均灰度比较,差异有统计学意义(F=19.912,P=0.000)。进一步两两比较,模型组平均灰度大于空白对照组、中药对照组及腰腿痛胶囊组(P=0.000;P=0.000;P=0.000);腰腿痛胶囊组平均灰度小于中药对照组(P=0.000)。②降钙素基因相关肽平均灰度。4组大鼠降钙素基因相关肽平均灰度比较,差异有统计学意义(F=16.367,P=0.000)。进一步两两比较,模型组平均灰度大于空白对照组(P=0.000);腰腿痛胶囊组与模型组比较,差异无统计学意义(P=0.749);中药对照组大于模型组和腰腿痛胶囊组(P=0.033,P=0.000)。结论:腰腿痛胶囊通过降低实验性腰椎间盘突出症大鼠背根神经节中P物质含量,减轻由髓核组织对背根神经节的损伤和刺激而引起的疼痛,这可能是腰腿痛胶囊治疗腰椎间盘突出症的作用机理之一。
Objective：To observe the effect of capsules for lumbago-leg pain（CLLP） on the contents of substance P（SP） and calcitouin gene-related peptide（CGRP） in dorsal root ganglia of rats models with lumbar disc herniation （LDH）in nucleus pulposus transplantation type, and to explore the mechanism of capsules for lumbago-leg pain in the treatment of LDH. Methods： Forty-eight adult SD rats were randomly divided into blank control group,model group,traditional Chinese medicine（TCM） control group and CLLP group, 12 cases in each group. All the rats were administrated with modeling method of nucleus pulposus transplantation to build LDH rats models except those in the blank control group. From the second day after modeling, rats in CLLP group were administrated with suspension composed of CLLP and normal saline per day, cases in TCM control group were administrated with suspension composed of pills for lumbago-leg pain and normal saline per day, while the others in blank control group and model group were administrated with normal saline in the same volumes. After coutinious 2 weeks of intragastric administration, caudal nucleus pulposus of rats were transplanted in dorsal root ganglia of spinal nerve near the L4 - L6 ,then SP and CGRP contents in dorsal root ganglia for all the rats were measured through using immunohistochemistry. Results： There were statistical differences in average gray scale of SP among the 4 groups （F = 19. 912, P = 0. 000 ）. Average gray scale of SP in model group was higher than that of blank control group, TCM control group and CLLP group respectively （ P = 0. 000 ; P = 0. 000 ; P = 0. 000） ;and the average gray scale of SP in CLLP group was lower than that of TCM control group（ P = 0.00（3）. There were statistical differences in average gray scale of CGRP among the 4 groups （F = 16. 367, P = 0. 000）. Average gray scale of CGRP of model group was higher than that of blank control group （ P = 0. 000 ） ; and there was no statistical difference between CLLP group and model group （ P = 0. 749） ;and the average gray scale of CGRP of TCM control group was higher than that of model group and CLLP group respectively （P = 0. 033 ,P = 0. 000 ）. Conclusion：The capsule for lumbago-leg pain can relieve the pain caused by the injuries and stimulations of dorsal root ganglia from nucleus pulposus through reducing SP contents in dorsal root ganglia of experimental LDH rats. It may be one of the mechanisms of action of the capsule for lumbago-leg pain in the treatment of LDH.