文献类型：期刊文献

中文题名：不同音乐速度对轻度认知障碍大鼠海马CA1区神经编码及SYP、GAP-43蛋白表达的影响

英文题名：Effects of Different Music Velocities on Neural Coding And Expressions ofSYP and GAP-43 Proteins in Hippocampal CA1 Region in Rats with Mild Cognitive Impairment

作者：刘安国[1];吴艳辉[1];郝小路[1];郑晓琳[1];周娜[2];王觉[1];火霞丽[1];严兴科[1]

第一作者：刘安国

机构：[1]甘肃中医药大学针灸推拿学院,甘肃兰州730000;[2]西宁市湟中区第一人民医院,青海西宁810000

第一机构：甘肃中医药大学针灸推拿学院

年份：2026

卷号：53

期号：6

起止页码：227

中文期刊名：辽宁中医杂志

外文期刊名：Liaoning Journal of Traditional Chinese Medicine

收录：;北大核心:【北大核心2023】；

基金：国家自然科学基金项目(82260965);甘肃省自然科学基金项目(22JR5RA583);“岐黄英才”导师专项基金项目(ZYXKSD-202220);甘肃省第三批陇原青年英才项目(省委人才小组发[2024]11号);甘肃省委组织部重点人才项目(甘组遵字[2022]77号)。

语种：中文

中文关键词：音乐疗法;轻度认知障碍;放电频率;突触蛋白;生长相关蛋白

外文关键词：music therapy;mild cognitive impairment;discharge frequency;synaptic proteins;growth-related proteins

摘要：目的探究不同速度音乐对轻度认知障碍(mild cognitive impairment,MCI)模型大鼠海马CA1区神经元自发放电规律以及突触素蛋白(synaptophysin protein,SYP)、生长相关蛋白-43(growth-related protein,GAP-43)表达的影响,为音乐疗法干预MCI的效应机制和应用研究提供依据。方法45只SPF级雌性SD大鼠,随机数字表分为空白组、模型组、80 BPM组、104 BPM组、128 BPM组,每组9只。除空白组给予每天喂食喂水、搬取鼠笼外,不接受任何干预,其余大鼠颈背部皮下注射D-半乳糖(1000 mg·kg^(-1)·d^(-1))连续7 d进行MCI模型复制,并以Morris水迷宫实验验证模型。再以80、104、128 BPM的不同速度莫扎特K.448音乐干预各治疗组,每天2 h,持续6周。治疗结束时,再次进行水迷宫实验观察各组大鼠学习记忆能力。结束后,通过Cerebus多通道数据采集系统采集并分析各组大鼠海马CA1区神经元自发放电光栅图、平均放电频率、峰-峰间期(inter-spike interval,ISI)、时频分布,并使用免疫组织化学技术分析其SYP、GAP-43蛋白AOD值的变化。结果干预结束后,与空白组比较,模型组大鼠Morris水迷宫逃避潜伏期明显增加(P<0.05)、原平台区域滞留时间和穿越次数均明显减少(P<0.05),海马CA1区自发放电光栅图稀疏、平均放电频率降低(P<0.05)、ISI增大(P<0.05),时频分布不均且能量最低,SYP蛋白AOD值明显下降(P<0.05),GAP-43蛋白AOD值明显上升(P<0.05);与模型组比较,3个音乐治疗组大鼠逃避潜伏期减少(P<0.05)、原平台区域滞留时间增加(80 BPM组明显增加,P<0.05)、穿越平台次数明显增加(除128 BPM组,P<0.05),海马CA1区放电光栅图变密、平均放电频率增高(P<0.05)、ISI缩小(P<0.05),时频分布较均匀,能量较高,SYP蛋白AOD值明显上升(除128 BPM组,P<0.05),GAP-43蛋白AOD值明显下降(除128 BPM,P<0.05);3个音乐治疗组之间比较,104 BPM组、128 BPM组大鼠逃避潜伏期明显增加(P<0.05),80 BPM组光栅条带最密,128 BPM组放电频率明显降低(P<0.05),80 BPM组大鼠学习记忆能力各项指标改善效应最佳(P<0.05)。结论MCI模型大鼠存在学习记忆能力下降,以及大脑海马CA1区神经电生理紊乱,并且突触相关蛋白SYP、GAP-43表达异常的现象。80 BPM音乐可显著改善MCI大鼠学习记忆能力,其疗效机制可能与调节海马CA1区神经元异常放电编码,促进突触相关蛋白表达有关。
Objective To investigate the effects of music of different velocities on the spontaneous emission of neurons in the CA1 region of the hippocampus and the expression of synaptophysin protein(SYP)and growth-related protein(GAP-43)in the rats with mild cognitive impairment(MCI)model.Methods A total of 45 SPF-level female SD rats were randomly divided into blank group,model group,80 BPM(music speed)group,104 BPM group and 128 BPM group,with 9 rats in each group.Except for the blank group,which was fed daily with water and the mouse cage was removed,no intervention was received,and the rest of the rats were injected subcutaneously with D-galactose(1000 mg·kg^(-1)·d^(-1))on the back of the neck for 7 consecutive days to replicate the MCI model,and the model was verified by Morris water maze experiment.Mozart K.448 music at different speeds of 80,104,128 BPM was used to intervene in each treatment group for two hours per day for six weeks.At the end of treatment,the water maze experiment was performed again to observe the learning and Xi memory ability of the rats in each group.At the end,the cerebus multi-channel data acquisition system was used to collect and analyze the spontaneous electrical grating diagram,average discharge frequency,inter-spike interval(ISI)and time-frequency distribution of neurons in the hippocampus CA1 region of each group,and the changes of AOD values of SYP and GAP-43 proteins were analyzed by immunohistochemistry method.Results Compared with those in the blank group,the incubation period of Morris water maze evasion in the model group increased significantly(P<0.05),the residence time and crossing times in the original platform area were significantly reduced(P<0.05),the spontaneous electric grating pattern in the CA1 region of the hippocampus was sparse,the average discharge frequency decreased(P<0.05),the ISI increased(P<0.05),the time-frequency distribution was uneven and the energy was the lowest,the AOD value of SYP protein decreased significantly(P<0.05),and the AOD value of GAP-43 protein increased significantly(P<0.05).Compared with those in the model group,the rats in the three music therapy groups had a decrease in the escape latency(P<0.05),an increase in the residence time in the original platform area(a significant increase in the 80BPM group,P<0.05),a significant increase in the number of platform crossings(except for the 128 BPM group,P<0.05),a denser of the HIPOCAMPAL CA1 area discharge grating pattern,an increase in the average discharge frequency(P<0.05),a decrease in ISI(P<0.05),a more uniform time-frequency distribution and higher energy,a significant increase in the AOD value of SYP protein(except for the 128 BPM group,P<0.05),and a significant decrease in the AOD value of GAP-43 protein(except 128BPM,P<0.05).The escape latency of the rats in the 104 BPM group and the 128 BPM group increased significantly(P<0.05),the 80 BPM group had the densest grating band,the discharge frequency in the 128 BPM group decreased significantly(P<0.05),and the 80 BPM group had the best improvement effect on learning and memory ability(P<0.05).Conclusion In the MCI model,there was a decrease in memory ability,neuroelectrophysiological disorders in the CA1 region of the hippocampus,and abnormal expressions of synapse-related proteins SYP and GAP-43.80 BPM music can significantly improve the learning and memory ability of MCI rats,and its therapeutic mechanism may be related to the regulation of abnormal firing coding of neurons in the hippocampus CA1 region and the promotion of synapse-associated protein expression.