文献类型：期刊文献

英文题名：Therapeutic potential of Traditional Chinese Medicine Yisui Shengxue pills ((sic)(sic)(sic)(sic)(sic)) to inhibit hypoxia-inducible factor-1alpha and general control nonderepressible 2 to regulate the post-chemotherapy immune response: integrating network pharmacology and experimental validation

作者：Qi, Yafeng[1];Liu, Yu[1];Liu, Yeyuan[1];Li, Yangyang[1];Zhang, Shangzu[1];Chen, Yaping[1];Xu, Qian[1];Hao, Guoxiong[1];Liu, Yongqi[3];Zhang, Liying[2];Zhang, Zhiming[4]

第一作者：Qi, Yafeng

通信作者：Zhang, LY[1];Zhang, ZM[2]

机构：[1]Gansu Univ Tradit Chinese Med, Clin Sch Tradit Chinese Med, Lanzhou 730000, Peoples R China;[2]Gansu Univ Chinese Med, Sch Tradit Chinese & Western Med, Lanzhou 730000, Peoples R China;[3]Gansu Univ Tradit Chinese Med, Sch Basic Med, Lanzhou 730000, Peoples R China;[4]Gansu Prov Hosp Tradit Chinese Med, Dept Oncol, Lanzhou 730050, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Sch Tradit Chinese & Western Med, Lanzhou 730000, Peoples R China;[2]corresponding author), Gansu Prov Hosp Tradit Chinese Med, Dept Oncol, Lanzhou 730050, Peoples R China.|[10735]甘肃中医药大学;

年份：2025

卷号：45

期号：5

起止页码：1087

外文期刊名：JOURNAL OF TRADITIONAL CHINESE MEDICINE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001594490400001)】；

基金：This research was supported and assisted by Gansu University of Traditional Chinese Medicine, Gansu Provincial Cancer Clinical Research Center of Integrated Traditional Chinese and Western Medicine, Gansu Provincial Key Laboratory of Molecular Medicine and Traditional Chinese Medicine Prevention and Control of Major Diseases in Colleges and Universities, Chinese Academy of Sciences Dunhuang Provincial and Ministerial Key Laboratory of Medicine and Translation, and the National Studio of famous Chinese medicine practitioner Zhang Zhiming. The authors would like to express their gratitude to EditSprings (https:// www.editsprings.cn) for the expert linguistic services provided.

语种：英文

外文关键词：drug therapy; immunosuppression therapy; myeloid-derived suppressor cells; post-chemotherapy; network pharmacology; Yishui Shengxue pills

摘要：OBJECTIVE: To investigate the efficacy and mechanism of Traditional Chinese Medicine Yishui Shengxue pills ((sic) (sic) (sic) (sic) (sic) , YSSX) in mouse models of immunosuppression induced by three chemotherapy drugs. METHODS: We determined an optimal intervention dose of YSSX to investigate efficacy. Changes in immune cell subpopulations were detected by flow cytometry, while immunofluorescence, immunohistochemistry, and other molecular biology methods, were used to verify pathway targets. We used PX-478, an inhibitor of hypoxiainducible factor-1 alpha (HIF-1 alpha), to validate the mechanism of action.RESULTS: Analysis showed that YSSX enhanced the immunity of mouse models of immunosuppression. At the cellular level, YSSX reduced the numbers of myeloid-derived suppressor cells (MDSCs) and enhanced CD8+ T cell infiltration. At the molecular level, YSSX reduced the expression levels of hypoxia-inducible factor-1 alpha (HIF-1 alpha), inducible nitric oxide synthase (iNOS), general control nonderepressible 2 (GCN2), and eukaryotic initiation factor 2 alpha (eIF2 alpha) in mouse MDSCs, thereby reducing the transcription of HIF-1 alpha, GCN2, and eIF2 alpha mRNA. Collectively, these changes led to the increased secretion of interferon-gamma and interleukin 12, concomitant with a reduction in tumor necrosis factor-alpha level. CONCLUSIONS: YSSX improved MDSC-mediated immunosuppression in a mouse model after chemotherapy by inhibiting the HIF-1 alpha/iNOS-GCN2/eIF2 alpha signaling axis. (c) 2025 JTCM. All rights reserved.