文献类型：期刊文献

英文题名：Human-mouse chimeric brain models constructed from iPSC-derived brain cells: Applications and challenges

作者：Zhao, Ya[1];Liu, Ke[1,2];Wang, Yinghua[3];Ma, Yifan[1,2];Guo, Wenwen[1];Shi, Changhong[1]

第一作者：Zhao, Ya

通信作者：Shi, CH[1]

机构：[1]Fourth Mil Med Univ, Lab Anim Ctr, Xian 710032, Shaanxi, Peoples R China;[2]Gansu Univ Tradit Chinese Med, Lanzhou 730030, Peoples R China;[3]Yanan Univ, Med Coll, Yanan 716000, Peoples R China

第一机构：Fourth Mil Med Univ, Lab Anim Ctr, Xian 710032, Shaanxi, Peoples R China

通信机构：[1]corresponding author), Fourth Mil Med Univ, Lab Anim Ctr, Xian 710032, Shaanxi, Peoples R China.

年份：2024

卷号：379

外文期刊名：EXPERIMENTAL NEUROLOGY

收录：;Scopus(收录号：2-s2.0-85195540701);WOS:【SCI-EXPANDED(收录号:WOS:001253284400001)】；

基金：The work was supported by Laboratory Animal Foundation Program of Military (No. SYDW[2018]01; SYDW[2020]20) , and the Shaanxi Province Innovation Capability Support Plan (2022PT-38; 2022PT-42) .

语种：英文

外文关键词：Human induced pluripotent stem cell; Chimeric mouse model; Brain cells; Differentiate; Integration; Function

摘要：The establishment of reliable human brain models is pivotal for elucidating specific disease mechanisms and facilitating the discovery of novel therapeutic strategies for human brain disorders. Human induced pluripotent stem cell (iPSC) exhibit remarkable self-renewal capabilities and can differentiate into specialized cell types. This makes them a valuable cell source for xenogeneic or allogeneic transplantation. Human-mouse chimeric brain models constructed from iPSC-derived brain cells have emerged as valuable tools for modeling human brain diseases and exploring potential therapeutic strategies for brain disorders. Moreover, the integration and functionality of grafted stem cells has been effectively assessed using these models. Therefore, this review provides a comprehensive overview of recent progress in differentiating human iPSC into various highly specialized types of brain cells. This review evaluates the characteristics and functions of the human-mouse chimeric brain model. We highlight its potential roles in brain function and its ability to reconstruct neural circuitry in vivo. Additionally, we elucidate factors that influence the integration and differentiation of human iPSC-derived brain cells in vivo. This review further sought to provide suitable research models for cell transplantation therapy. These research models provide new insights into neuropsychiatric disorders, infectious diseases, and brain injuries, thereby advancing related clinical and academic research.