文献类型：期刊文献

英文题名：The H2Valdien derivatives regulate the epithelial-mesenchymal transition of hepatoma carcinoma cells through the Hedgehog signaling pathway

作者：Zhao, Xuhui[2];Shao, Xiangxiang[2];Huang, Xiaomin[2];Dang, Chunyan[1];Wang, Ruilin[2];Li, Hongling[1]

第一作者：赵小红

通信作者：Li, HL[1]

机构：[1]Gansu Prov Hosp, Dept Oncol, Lanzhou 730000, Peoples R China;[2]Gansu Univ Tradit Chinese Med, Clin Med Coll 1, Lanzhou 730000, Peoples R China

第一机构：Gansu Prov Hosp, Dept Oncol, Lanzhou 730000, Peoples R China

通信机构：[1]corresponding author), Gansu Prov Hosp, Dept Oncol, Lanzhou 730000, Peoples R China.

年份：2024

卷号：19

期号：1

外文期刊名：OPEN MEDICINE

收录：;Scopus(收录号：2-s2.0-85196752066);WOS:【SCI-EXPANDED(收录号:WOS:001251221700001)】；

基金：This work was supported by the National Natural Science Foundation of China (81760537)and the National Health Commission Key Laboratory of Gastrointestinal Tumour Diagnosis and Treatment 2022 Master/Postdoctoral Fund Project (NHCDP2022005).

语种：英文

外文关键词：H(2)Valdien derivatives; Hh signaling pathway; EMT; hepatoma carcinoma

摘要：This research delves into the influence of H2Valdien derivatives on the proliferation, migration, and apoptosis induction in hepatoma carcinoma cells (HepG2, Huh-7, and SMMC-7721), with a specific emphasis on inhibiting epithelial-mesenchymal transition (EMT) through modulation of the Hedgehog (Hh) signaling pathway. Utilizing the cell counting kit-8 method, flow cytometry, TUNEL assay, wound healing, and transwell assays, we observed a dose-dependent growth arrest and apoptosis induction in HepG2, Huh-7, and SMMC-7721 cells. Notably, H2Valdien derivatives exhibited a capacity to reduce migration and invasion, impacting the expression of EMT-associated proteins such as N-cadherin, vimentin, and E-cadherin. Mechanistically, these derivatives demonstrated the inhibition of the Hh signaling pathway by inactivating Sonic Hh (Shh) and smoothened proteins. This study underscores the robust antiproliferative and apoptosis-inducing effects of H2Valdien derivatives on hepatoma carcinoma cells and elucidates their regulatory role in EMT through modulation of the Hh signaling pathway, providing valuable insights for potential therapeutic interventions.