文献类型：期刊文献

中文题名：当归对血虚便秘模型小鼠的治疗作用及机制研究

英文题名：The therapeutic effect of Angelica sinensis on mice with constipation due to blood deficiency and its possible mechanism

作者：刘五州[1];妥海燕[1];杜丽东[1,2];牛亭惠[1];吴国泰[1,2];任远[1,2]

第一作者：刘五州

机构：[1]甘肃中医药大学药学院;[2]甘肃省中药药理与毒理学重点实验室

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2016

卷号：0

期号：2

起止页码：1

中文期刊名：甘肃中医学院学报

基金：甘肃省自然科学基金项目(1212RJZA079);甘肃省中药药理与毒理学重点实验室开放基金项目(ZDSYS-KJ-2013-006)

语种：中文

中文关键词：血虚便秘;小鼠;当归;治疗作用;红细胞;血红蛋白;小肠推进率;脾脏指数

外文关键词：constipation due to blood deficiency;;mice;;Angelica sinensis;;therapeutic effect;;RBC;;HGB;;gastric emptying rate;;spleen index

摘要：目的观察当归水煎液对血虚便秘小鼠的治疗作用,并初步探讨其作用机制。方法将小鼠随机分为正常对照组,模型对照组,阳性对照组,当归水煎液高、低剂量组5组,以放血联合灌胃洛哌丁胺建立小鼠血虚便秘模型,阳性对照组灌胃润肠丸混悬液(0.75 g/kg),当归水煎液高、低剂量组灌胃当归水煎液(生药剂量分别为4.00,1.00 g/kg),正常对照组、模型对照组灌胃等体积蒸馏水,1次/d,连续2周。末次灌胃后眼眶采血,测定红细胞计数(RBC)和血红蛋白(HGB);处死小鼠,摘取肠管测定小鼠小肠推进率,摘取脾脏计算脾脏指数。结果与模型对照组比较,当归水煎液高、低剂量组外周血RBC和HGB显著升高,脾脏指数显著降低,同时小肠推进功能明显改善,组间比较差异有高度统计意义(P<0.01)。结论当归水煎液能明显改善血虚便秘模型小鼠的血虚证候,促进肠道蠕动。
Objective To observe the therapeutic effect of Angelica sinensisdecoction on mice with conspita-tion due to blood deficiency and explore its possible mechanism.Methods The mice were divided into controlgroup,model group,positive control group,low-dose treatment group and high-dose treatment group. Model micewere prepared by bloodletting and intragastric administration of loperamide. The positive control group was given thesuspension of Runchang Wan intragastrically. The low-dose treatment group and high-dose treatment group weregivenAngelica sinensisdecoction with the dosage of 4. 00 g/kg and 1. 00 g/kg respectively. The other two groupstook equivalent distilled water. All mice were treated once a day and lasted for 2 weeks. After the last treatment,blood was drawn from orbit. RBC and HGB were determined. The rate of gastric emptying and spleen index wereworked out.Results Compared with control groups,RBC and HGB of peripheral blood of mice in the treatmentgroup increased obviously,while the spleen index decreased. The intestinal propulsive movement improved clearly.There was a significant difference(P<0. 01).Conclusion Angelica sinensisdecoction can alleviate the symptomof blood deficiency in mice and promote their intestine movements.