文献类型：期刊文献

中文题名：银杏叶提取物对佐剂性关节炎大鼠血清抗氧化能力及肿瘤坏死因子含量的影响

英文题名：Study on the effects of Ginkgo biloba extract on the antioxidation ability and the serum TNF-α contents in adjuvant arthritis rats

作者：刘继新[1];刘成松[2]

第一作者：刘继新

机构：[1]甘肃省人民医院药剂科;[2]甘肃中医学院附属医院药剂科

第一机构：甘肃省人民医院药剂科

年份：2012

卷号：32

期号：8

起止页码：607

中文期刊名：中国医院药学杂志

外文期刊名：Chinese Journal of Hospital Pharmacy

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：银杏叶提取物;佐剂性关节炎;抗氧化作用;肿瘤坏死因子

外文关键词：Ginkgobiloba extract; adjuvant arthritis; antioxidation; TNFα

摘要：目的:考察银杏叶提取物(EGb761)对佐剂性关节炎(AA)大鼠血清抗氧化能力及肿瘤坏死因子(TNF-α)含量的影响,初步探讨银杏叶提取物治疗佐剂性关节炎的作用机制。方法:雄性SD大鼠于右后足跖皮内注射弗氏完全佐剂(FCA)0.1 mL建立大鼠佐剂性关节炎模型。致炎第12天分别应用EGb761低、中、高剂量(50,100,200 mg.kg-1)灌胃对其进行治疗,连续给药16 d;并以雷公藤多苷(40 mg.kg-1)作为阳性对照。采用足容积法测量继发侧足肿胀度。致炎第28天处死大鼠,收集血清,检测血清中髓过氧化物酶(MPO)、超氧化物歧化酶(SOD)和一氧化氮合酶(iNOS)活力、丙二醛(MDA)、一氧化氮(NO)及TNF-α含量。结果:致炎后第12天,大鼠继发性关节炎出现,给予不同剂量的EGb761(50,100,200 mg.kg-1),从致炎后第20天开始,EGb761可明显减轻继发性AA大鼠足爪的肿胀度;与模型对照组比较,EGb761各剂量组大鼠血清中MPO、iNOS活力及MDA、NO和TNF-α含量均明显降低(P<0.05或P<0.01),而SOD含量明显升高(P<0.01)。结论:EGb761对AA大鼠继发性炎症具有显著的治疗作用,其作用机制可能与提高AA大鼠血清抗氧化能力,抑制致炎因子TNF-α生成有关。
OBJECTIVE To investigate the antioxidation ability of Ginkgo biloba extract （EGb761） on adjuvant arthritis （AA） in rats and to probe into its underlying mechanism. METHODS The AA was induced by Freund＇s complete adjuvant （FCA） in male SD rats. Rats were intragastrically administered different doses of EGb761 （50, 100, and 200 mg·kg^-1） on the 12th day after injection of FCA, the drugs were given for 16 days. Tripterygium wilfordii ployglycosidium （TPT） at 40 mg ·kg^-1 was used as a positive control drug. Rats were killed after FCA was injected on the 28th days. The activities of myeloperoxidase （MPO）, superoxide dismutase （SOD）, inducible nitric oxide synthase （iNOS）, and the contents of malonyldialdehyde （MDA） and nitric oxide （NO） in serum were detected by biochemistry methods. The contents of TNF-α in serum were measured by enzyme-linked immunosorbent assay methods. RESULTS The secondary inflammation of AA rats appeared on the 12th day after injection of FCA. At the same time （d 12）, different doses EGb761 were given to AA rats. It was found that EGb761 could significantly inhibit the secondary paw swelling of AA rats from the 20th day. Compared with the model group, the activities of MPO and iNOS and the contents of MDA, NO and TNF-α in serum were significantly decreased （P〈0. 05 or P〈0. 01 ） ; the activities of SOD in serum were increased （P〈0. 05 or P〈0. 01）. CONCLUSION EGb761 can alleviate the inflammatory reactions in AA through inhibiting oxygen free reaction, exerting antioxidation effects and decreasing the produc tion of TNF-α.