文献类型：期刊文献

中文题名：补肾调泡周期法对多囊卵巢模型大鼠卵巢AMH及AMHR Ⅱ表达的调控

英文题名：The Regulation of Bushen-Follicle Recuperation Therapy on Expression of AMH Protein and Receptor in PCO Model Rats

作者：陈乐[1];杨硕[2];尤昭玲[3];杨永琴[4];朱旭[2]

第一作者：陈乐

机构：[1]湖南中医药大学,湖南长沙410208;[2]湖南中医药大学中西医结合学院,湖南长沙410208;[3]湖南中医药大学第一附属医院,湖南长沙410007;[4]甘肃中医药大学,甘肃兰州730000

第一机构：湖南中医药大学,湖南长沙410208

年份：2019

卷号：30

期号：11

起止页码：1314

中文期刊名：中药新药与临床药理

外文期刊名：Traditional Chinese Drug Research and Clinical Pharmacology

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：国家自然科学基金资助项目（81503611）;湖南省自然科学基金资助项目（2015JJ6078）;湖南省教育厅优秀青年基金资助项目（15B180）

语种：中文

中文关键词：补肾调泡周期法;AMH;AMHRⅡ;PCO大鼠;卵泡发育

外文关键词：Bushen-follicle recuperation therapy;AMH;AMHRⅡ;PCO rat model;follicular development.

摘要：目的研究补肾调泡周期法对多囊卵巢(PCO)模型大鼠卵巢抗苗勒氏管激素(AMH)及Ⅱ型受体(AMHR Ⅱ)蛋白表达的调控,初步探讨补肾调泡周期法治疗多囊卵巢综合征(PCOS)的作用机制。方法110只SD雌性大鼠随机选取10只作为正常组,剩余100只采用来曲唑溶液灌胃法构建PCO大鼠模型成功后,设立模型组、阳性对照组、A-逍遥散(高、低剂量)组、B-三子汤(高、低剂量)组、A+B-逍遥散+三子汤周期用药4组,每组10只。统计分析各组大鼠治疗前后体质量增加量;卵巢脏器系数;HE染色法观察卵巢组织病理形态;免疫组织化学法检测PCO大鼠卵巢AMH、AMHRⅡ蛋白表达。结果体质量增加量:与正常组比较,PCO模型组大鼠体质量增加量明显增多(P<0.01);与模型组比较,A低B高组、B低组及阳性对照组大鼠体质量增加量减少(P<0.05)。卵巢系数:与正常组比较,PCO模型组大鼠卵巢系数明显升高(P<0.01);与模型组比较,A低组大鼠卵巢系数降低(P<0.05),A高组、B高组、A低B低组、A低B高组及阳性对照组大鼠卵巢系数明显降低(P<0.01)。AMH蛋白表达:与正常组比较,PCO模型组大鼠卵巢AMH蛋白表达明显升高(P<0.01);与模型组比较,所有治疗组及阳性对照组大鼠卵巢AMH蛋白表达明显降低(P<0.01);治疗组间两两比较:与A高B高组比较,A(高、低)组、B(高、低)组、A低B低组、A低B高组大鼠卵巢AMH蛋白表达明显升高(P<0.01),A高B低组大鼠卵巢AMH蛋白表达升高(P<0.05)。AMHRⅡ蛋白表达:与正常组比较,PCO模型组大鼠卵巢组织AMHRⅡ蛋白表达明显升高(P<0.01);与模型组比较,A低剂量组AMHRⅡ蛋白表达降低(P<0.05),余治疗组及阳性对照组AMHRⅡ蛋白表达明显降低(P<0.01);治疗组间两两比较:与B高组比较,A低组、B低组大鼠卵巢组织AMHRⅡ蛋白表达明显升高(P<0.01),A高组大鼠卵巢组织AMHRⅡ蛋白表达升高(P<0.05),A高B高组AMHRⅡ蛋白表达明显降低(P<0.01);与B低组比较,B高组、A+B周期用药4组及阳性对照组AMHRⅡ蛋白表达明显降低(P<0.01);与A低B低组比较,A(低、高)组、B低组、A高B低组AMHRⅡ蛋白表达明显升高(P<0.01);与A低B高组比较,A(低、高)组、B低组AMHRⅡ蛋白表达明显升高(P<0.01),A高B高组AMHRⅡ蛋白表达明显降低(P<0.01),A高B低组AMHRⅡ蛋白表达降低(P<0.05);与A高B高组比较,A(低、高)组、B(低、高)组、A低B高组、A高B低组AMHRⅡ蛋白表达明显升高(P<0.01);A+B周期用药4组间大鼠卵巢AMHRⅡ蛋白表达差异比较:A高B高组Objective To study the regulation of Bushen-follicle recuperation therapy on the expression of AMH Protein and type Ⅱ receptor(AMHRⅡ)expression in PCO rat models,to investigate the mechanism of Bushenfollicle recuperation therapy for polycystic ovary syndrome(PCOS).Methods Ten of 110 female SD rats were randomly selected as normal group,the remained 100 rats were divided into model group,positive control group,A-Xiaoyao pulvis high-,and low-dose groups,B-Sanzi decoction high-,and low-dose groups,A+B-Xiaoyao pulvis+Sanzi decoction group,10 rats in each group.The increment of body mass was statistically analyzed.Ovarian organ coefficient were measured.The pathological morphology of ovarian tissue was observed by HE staining.AMH and AMHRⅡprotein expression in PCO rats’ovarian were detected by immunohistochemisty method.Results Body mass increment:compared with the normal group,the body mass increment of PCO model group was increased significantly(P<0.01).Compared with the model group,the body mass increment of A low+B high group,B low group and positive control group were reduced(P<0.05).Ovarian coefficient:compared with the normal group,the ovarian coefficient of PCO model group was increased significantly(P<0.01).Compared with the model group,the ovarian coefficient of A low group reduced(P<0.05),and those of A high group,B high group,A low+B low group,A low+B low group,and the positive control group reduced significantly(P<0.01).AMH protein expression:compared with the normal group,the expression of AMH protein in the ovaries of PCO model group was increased significantly(P<0.01).Compared with the model group,the expression of AMH protein in ovary of all the treatment groups and positive control groups were reduced significantly(P<0.01).Comparison between treatment groups:compared with A high+B high group,the expression of AMH protein in the ovary of rats in A high and low groups,B high and low groups,A low+B low group,and A low+B high group were increased significantly(P<0.01),and the expression of AMH protein in the ovaries of rats in A high+B low group were increased significantly(P<0.05).AMHRⅡprotein expression:compared with normal group,AMHRⅡprotein expression of the PCO model group rats’ovarian tissue were increased significantly(P<0.01).Compared with model group,AMHRⅡprotein expression of A low dose group was reduced(P<0.05),those of the other treatment groups and positive control group were reduced significantly(P<0.01).Comparison between treatment group:compared with group B high group,AMHRⅡprotein expression of A low group and B low group rats’ovarian tissues were increased significantly(P<0.01),that of A high group rats’ovarian tissue were increased(P<0.05);AMHRⅡprotein expression of A high+B high group was reduced significantly(P<0.01).Compared with B low group,AMHRⅡprotein expression of B high group,A+B cycle groups and positive control group significantly were reduced(P<0.01).Compared with A low+B low group,AMHRⅡprotein expression of A low and high groups,B low group,A high+B low group were increased significantly(P<0.01).Compared with A low+B high group,AMHRⅡprotein expression of A low and high groups,B low group were increased significantly(P<0.01),that of A high+B high group were reduced significantly(P<0.01),and that of A high+B low group were reduced(P<0.05).Compared with A high+B high group,AMHRⅡprotein expression of A low and high groups,and B low and high groups,A low+B high,A high+B low group were increased significantly(P<0.01).Comparison of AMHRⅡprotein expression between A+B cycle groups:A high+B high