文献类型：期刊文献

中文题名：氧化苦参碱对慢性乙型肝炎外周血单个核细胞Toll样受体7信号通路的影响

英文题名：Effect on Toll-Like Receptor 7 Signaling Pathway in PBMCs of Chronic Hepatitis B Patients by Oxymatrine in vitro

作者：姚凝[1];王昕[2]

第一作者：姚凝

机构：[1]甘肃中医药大学临床医学院,甘肃兰州730000;[2]甘肃中医药大学中西医结合学院,甘肃兰州730000

第一机构：甘肃中医药大学临床医学院

年份：2018

卷号：36

期号：5

起止页码：1196

中文期刊名：中华中医药学刊

外文期刊名：Chinese Archives of Traditional Chinese Medicine

收录：CSTPCD;;北大核心:【北大核心2017】；

基金：河南省医学科技攻关计划项目(201303231)

语种：中文

中文关键词：氧化苦参碱;toll样受体信号通路7;细胞因子;慢性乙型肝炎

外文关键词：Oxymatrine;toll - like receptor 7;cytokine;chronic hepatitis B

摘要：目的：分析氧化苦参碱治疗慢性乙型肝炎的作用机制。方法：外周血单个核细胞（PBMCs）分离提取,在体外与氧化苦参碱共孵育48 h,检测Toll样受体7（TLR7）信号通路主要级联分子基因表达水平;TLR7配体刺激经氧化苦参碱预处理的PBMCs 24 h,检测在配体诱导下抗病毒细胞因子分泌水平。结果：氧化苦参碱上调并活化PBMCs细胞中TLR7信号转导通路,在TLR7特异性配体R848刺激下分泌抗病毒细胞因子。结论：氧化苦参碱可上调并活化PBMCs中TLR7信号通路,有利于其发挥其抗CHB作用。
Objective： Investigate the immunomodulatory mechanism of Oxymatrine extracted from Sophora alopecuraides L. to anti-HBV in vitro. Methods： Human peripheral blood mononuclear cells（PBMCs） were isolated from heparinized whole blood from 48 chronic hepatitis B（CHB） patients. The PBMCs from each patient were divided into two groups according to pre-treatment with Oxymatrine or not in vitro. We examined the changes of expression and function of toll-like receptor 7（TLR7） signaling transduction pathway in the PBMCs with different treatment methods and we investigated the TLR7 ligand on antiviral cytokine secretions in vitro. Results： For the TLR7 signaling pathway,Oxymatrine not only augmented the expressions of TLR7 signaling transduction molecules,but also activated the TLR7 signal function.This study clearly demonstrated that TLR7 ligand could stimulate PBMCs that have been pre-treated with Oxymatrine.Furthermore,the quantity of antiviral cytokines secreted by the pre-treated PBMCs was greater than those without pre-treatment. Conclusion： This study suggests Oxymatrine could be a strong immunomodulator,influence TLR7 signaling transduction and improve the immune efficacy of TLR7 ligand against CHB. The data indicates that Oxymatrine possesses an anti-HBV activity and exerts its anti-virus effects via activation of TLR7 signal pathway.