文献类型：期刊文献

英文题名：Comparison of Liposome-Polycation-DNA(LPD) and Monophosphoryl Lipid A(MPL) Adjuvant Formulations in BALB/c Mice Models

作者：Zhang, Xiao-Ping[1];Cui, Wen-Hui[2]

第一作者：张小平

通信作者：Zhang, XP[1]

机构：[1]Gansu Coll Tradit Chinese Med, Lanzhou 730000, Gansu, Peoples R China;[2]Longnan Normal Coll, Longnan, Gansu, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Coll Tradit Chinese Med, Lanzhou 730000, Gansu, Peoples R China.|[10735]甘肃中医药大学;

年份：2012

卷号：41

期号：4

起止页码：356

外文期刊名：IMMUNOLOGICAL INVESTIGATIONS

收录：;Scopus(收录号：2-s2.0-84862567390);WOS:【SCI-EXPANDED(收录号:WOS:000302069800002)】；

语种：英文

外文关键词：Adjuvant; DNA adjuvant; Monophosphoryl Lipid A

摘要：It is of fundamental importance to use an appropriate adjuvant to generate a potent immune response for immunotherapy. In this study, we had a comparative investigation on the effectiveness of two adjuvant formulations, liposome-polycation-DNA (LPD) and monophosphoryl lipid A(MPL) in combination with a truncated peptide of bFGF(tbFGF) as antigen. LPD/tbFGF induced continuously increasing antibodies expression during the whole immunization period. In contrast, the level of antibodies was variable in MPL/tbFGF-immunized mice, MPL/tbFGF elicited potent antibodies response in the early-phase of immunization (during the first 3 immunizations), but the later immunizations did not produce a significant increase in the level of antibodies. Evaluation of IFN-gamma and IL-4 responses revealed that both LPD/tbFGF and MPL/tbFGF demonstrated generation of higher level of IFN-gamma, whereas no significant increase in IL-4 levels was detected in the two groups. In addition, histological analysis exhibited obvious germinal centers in the spleen tissues of LPD/tbFGF mice. The data suggested that LPD would be a promising long-effective adjuvant due to its potent and persistent immunostimulation and MPL could play an appropriate role in short-acting immunization.