文献类型：期刊文献

中文题名：平痫冲剂对幼年海人酸颞叶癫痫模型大鼠学习记忆行为的影响

英文题名：Effects of PingXian Granules on the Learning and Memory Behavior of Young Rats with Temporal Lobe Epilepsy

作者：孟陆亮[1,2];毋荣荣[1];史正刚[1];张加权[1];陈开兵[3];李永新[4]

第一作者：孟陆亮

机构：[1]甘肃中医学院;[2]渭南职业技术学院;[3]甘肃中医学院附属医院;[4]甘肃省中医院

第一机构：甘肃中医药大学

年份：2013

卷号：26

期号：4

起止页码：19

中文期刊名：西部中医药

外文期刊名：Western Journal of Traditional Chinese Medicine

收录：CSTPCD

基金：2010年甘肃省中医药英才基金项目(编号GZK-2010-61)

语种：中文

中文关键词：平痫冲剂;海人酸;颞叶癫痫,幼年;学习记忆行为;脑电图

外文关键词：PingXian granules; kainic acid; temporal lobe epilepsy;, young, learning and memory behavior, EEG

摘要：目的:建立幼年海人酸颞叶癫痫模型并给予平痫冲剂治疗,探讨对其学习记忆行为的影响。方法:将60只40日龄Wi st ar大鼠,随机分为正常对照组(A组)、模型组(B组)、阳性对照组(C组)及平痫冲剂低(D组)、中(E组)、高剂量组(F组)。除A组外,其余各组脑立体定位注射海人酸诱发颞叶癫痫。A组正常饲养,B组灌服等量生理盐水,D、E、F组灌服平痫冲剂,低、中、高给药剂量按人(70 kg)用药剂量的3倍、6倍、12倍给药,分别为0.7、1.4、2.8 g/(kg.d),C组灌服等量卡马西平,均每日灌胃1次,连续灌胃1月。实验过程中监测大鼠症状的改变,深部脑电图观察颞叶癫痫的病理过程及治疗前后的脑电变化,利用Morri s水迷宫和八臂迷宫实验评价各组大鼠的学习记忆行为。结果:①大鼠症状的改变:海人酸注射后半小时到6小时大鼠出现急性发作,进而逐渐缓减,2个月后出现自发发作。平痫冲剂治疗可抑制癫痫发作及其发展;②电生理学改变:脑电图于24小时和2个月后均表现为典型的癫痫发作电活动及发作间期表现。治疗后癫痫波持续时间缩短;③学习记忆行为的变化:海人酸颞叶癫痫大鼠学习记忆能力明显降低。平痫冲剂可缩短其逃避潜伏期,提高穿越原平台次数,减少参考记忆和工作记忆错误。结论:海人酸模型是模拟人类颞叶癫痫较为理想的动物模型;平痫冲剂对癫痫发作及脑电活动具有抑制作用,并且可以改善其学习记忆行为能力。
Objective： To establish the temporal lobe epilepsy models with kainic acid and explore the effects of PingXian granules on the learning and memory behavior of rats. Method： All 60 rats were randomized into normal control group （A）, model group （B）, positive control group （C）, low, middle and high doses groups of PingXian gran- ules （D, E and F）. Except A, others groups were injected with kainic acid to build models with temporal lobe epilep- sy. Rats in A group were fed normally, B group were given with the same amount of physiological saline, D, E and F were administered with PingXian granules in the doses of 0.7, 1.4 and 2.8 g/（kgod）, C received the same amounts of carbamazepine through intragastric administration, each day one time, for one consecutive month. The changes of the symptoms in rats were monitored, pathological courses of temporal lobe epilepsy and changes of EEG were ob- served before and after treating with depth EEG, learning and memory behavior of rats in groups were assessed with Morris water maze and eight-arm radial maze. Result： （1）Changes of the symptoms in rats： The rats were triggered a- cute attack in half an hour to six hours after injecting kainic acid, relieved gradually and attacked spontaneously in two months. PingXian granules could inhibit the onset and development of epilepsy; （2）Electrophysiological changes： EEG in 24 hours and two months showed typical electrical activity of seizure and attack performance. After treating the duration of epilepsy wave was shortened; （3）Changes of learning and memory behavior： Learning and memory behavior of temporal lobe epilepsy rats induced by kainic acid were decreased obviously. PingXian granules could shorten escape latency, raise the times of platform crossing and reduce the mistakes of reference memory and work- ing memory. Conclusion： Models established with kainic acid are ideal animals imitating human suffering from tem- poral lobe epilepsy; PingXian granules could inhibit the attack of epilepsy and electrical activity of brain, which could improve learning and memory ability.