文献类型：期刊文献

中文题名：雪松松针中总黄酮及4种指标成分的含量测定方法研究

英文题名：Content Determination Method of Total Flavonoids and 4 Maker Compounds in Pine Needles of Cedrus deodara

作者：胡鹏斌[1];韩涛[1];刘东彦[2];石晓峰[1,2];李师[1];宁红霞[1]

第一作者：胡鹏斌

机构：[1]甘肃中医学院,兰州730030;[2]甘肃省医学科学研究院,兰州730050

第一机构：甘肃中医药大学

年份：2015

卷号：26

期号：3

起止页码：381

中文期刊名：中国药房

外文期刊名：China Pharmacy

收录：CSTPCD;;北大核心:【北大核心2014】；

基金：甘肃省科技支撑计划项目(No.1204FKCA152)

语种：中文

中文关键词：雪松;松针;总黄酮;杨梅素;槲皮素;山柰酚;异鼠李素;含量测定;紫外分光光度法;高效液相色谱法

外文关键词：Cedrus deodara; Pine needles; Total flavonoids; Myrieetin; Quercetin; Campherol; Isorhamnetin; Content determination; UV spectrometry; HPLC

摘要：目的：建立测定雪松松针中总黄酮及杨梅素、槲皮素、山柰酚和异鼠李素含量的方法。方法：采用紫外分光光度法,以芦丁为对照品,以硝酸铝[Al（NO3）3]显色,在510 nm处测定雪松松针中总黄酮的含量。采用高效液相色谱法同时测定雪松松针中杨梅素、槲皮素、山柰酚和异鼠李素的含量。色谱柱为Agilert TC-C18,流动相为乙腈-0.1%磷酸水溶液（梯度洗脱）,流速为1.0 ml/min,检测波长为360 nm,柱温为30℃,进样量为20μl。结果：芦丁的含量在45.8~549μg范围内与吸光度呈良好线性关系（r=0.999 3）;平均加样回收率为97.65%,RSD=1.37%（n=6）。杨梅素、槲皮素、异鼠李素的质量浓度在1~10μg/ml、山柰酚的质量浓度在4~40μg/ml范围内与各自峰面积积分值呈良好线性关系（r≥0.999 2）,平均加样回收率分别为100.11%、97.63%、100.46%、102.11%,RSD分别为1.35%、1.49%、1.56%、1.51%（n=6）。雪松松针样品中总黄酮、杨梅素、槲皮素、山柰酚和异鼠李素的平均含量分别为81.9、0.229、0.503、0.843、0.297 mg/g。结论：本方法简便、准确、重复性好,可作为雪松松针中黄酮类物质的含量测定方法。
OBJECTIVE： To establish the method for the content determination of total flavonoids and myricetin, quercetin, campherol and isorhamnetin in pine needles of Cedrus deodara. METHODS ： Using rutin as control, the content of total flavonoids in pine needles of C. deodara was determined by UV spectrometry. The contents of myricetin, quercetin, campherol and isorhamnetin in C. deodara were determined by HPLC. The determination was performed on TC-C18 column with mobile phase consisted of acetonitrile-0.1% phosphoric acid solution （gradient elution） at the flow rate of 1.0 ml/min. The detection wavelength was set at 360 nm, and column temperature was 30 ℃. The sample size was 20 μl. RESULTS： The linear range was 45.8-549 μg for rutin（r= 0.999 3） with an average recovery of 97.65% （RSD= 1.37%, n=6）. The linear range were 1-10 μg/ml for myricetin, quercetin and isorhamnetin, and 4-40 μg/ml for kaempferol（r≥0.999 2）. The average recoveries of 4 maker compounds were 100.11% （RSD= 1.35% ）, 97.63% （RSD= 1.49% ）, 100.46% （RSD= 1.56% ） and 102.11% （RSD= 1.51% ）, respectively（n=6）. The total flavonoids and 4 maker compounds in pine needles of C. deodara were 81.9 mg/g, 0.229 mg/g, 0.503 mg/g, 0.843 mg/g and 0.297 mg/g, respectively. CONCLUSIONS： The method is simple, accurate and reproducible. It can be used for content determination of flavonoids in the pine needles of C. deodara. KEYWORDS Cedrus deodara; Pine needles; Total flavonoids; Myricetin; Quercetin; Campherol; Isorhamnetin; Content determination; UV spectrometry; HPLC