文献类型：期刊文献

英文题名：Establishment and characterization of a new intestinal-type ampullary carcinoma cell line, DPC-X3

作者：Chai, Changpeng[1,2];Miao, Xin[3,4];Su, Yuanhui[2];Yu, Cheng[2];Tang, Huan[2];Li, Lu[1];Wang, Zhengfeng[1,2];Yi, Jianfeng[5,6];Ye, Zhenzhen[2];Miao, Long[1,2];Zhang, Hui[2,7];Hu, Zhao[4,8];Chen, Luyang[4,8];Wu, Keren[4,8];Li, Ning[4,8];Wang, Linpei[9,11];Zhou, Wence[2,7];Xu, Hao[1,4,5,8,10]

第一作者：Chai, Changpeng

通信作者：Xu, H[1];Zhou, WC[2];Xu, H[3];Xu, H[4];Zhou, WC[5];Xu, H[6];Wang, LP[7];Xu, H[8];Wang, LP[9]

机构：[1]Lanzhou Univ, Dept Gen Surg 4, Hosp 1, Lanzhou 730000, Peoples R China;[2]Lanzhou Univ, Clin Med Coll 2, Lanzhou 730000, Peoples R China;[3]Zhejiang Chinese Med Univ, Affiliated Hosp 1, Zhejiang Prov Hosp Chinese Med, Affiliated Hosp 1, Hangzhou 310006, Peoples R China;[4]Zhejiang Chinese Med Univ, Sch Clin Med 1, Hangzhou 310006, Peoples R China;[5]Lanzhou Univ, Clin Med Coll 1, Lanzhou 730000, Peoples R China;[6]Gansu Univ Chinese Med, Sch Clin Med 1, Dept Surg, Lanzhou 730000, Peoples R China;[7]Lanzhou Univ, Dept Gen Surg, Hosp 2, Lanzhou 730000, Peoples R China;[8]First Affiliated Hosp Zhejiang Chinese Med Univ, Zhejiang Prov Hosp Chinese Med, Dept Orthoped Surg, Hangzhou 310006, Zhejiang, Peoples R China;[9]Fujian Med Univ, Dept Hepatobiliary & Pancreat Surg, Affiliated Hosp 2, Quanzhou 362000, Peoples R China;[10]Zhejiang Chinese Med Univ, Affiliated Hosp 1, Zhejiang Prov Hosp Chinese Med, Dept Hepatobiliary Surg, Hangzhou 310006, Zhejiang, Peoples R China;[11]Zhejiang Chinese Med Univ, Sch Clin Med 1, Dept Hepatobiliary Surg, 54 Youdian Rd, Hangzhou 310006, Zhejiang, Peoples R China

第一机构：Lanzhou Univ, Dept Gen Surg 4, Hosp 1, Lanzhou 730000, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Dept Gen Surg 4, Hosp 1, Lanzhou 730000, Peoples R China;[2]corresponding author), Lanzhou Univ, Clin Med Coll 2, Lanzhou 730000, Peoples R China;[3]corresponding author), Zhejiang Chinese Med Univ, Sch Clin Med 1, Hangzhou 310006, Peoples R China;[4]corresponding author), Lanzhou Univ, Clin Med Coll 1, Lanzhou 730000, Peoples R China;[5]corresponding author), Lanzhou Univ, Dept Gen Surg, Hosp 2, Lanzhou 730000, Peoples R China;[6]corresponding author), First Affiliated Hosp Zhejiang Chinese Med Univ, Zhejiang Prov Hosp Chinese Med, Dept Orthoped Surg, Hangzhou 310006, Zhejiang, Peoples R China;[7]corresponding author), Fujian Med Univ, Dept Hepatobiliary & Pancreat Surg, Affiliated Hosp 2, Quanzhou 362000, Peoples R China;[8]corresponding author), Zhejiang Chinese Med Univ, Affiliated Hosp 1, Zhejiang Prov Hosp Chinese Med, Dept Hepatobiliary Surg, Hangzhou 310006, Zhejiang, Peoples R China;[9]corresponding author), Zhejiang Chinese Med Univ, Sch Clin Med 1, Dept Hepatobiliary Surg, 54 Youdian Rd, Hangzhou 310006, Zhejiang, Peoples R China.

年份：2024

卷号：24

期号：1

外文期刊名：BMC CANCER

收录：;Scopus(收录号：2-s2.0-85212694645);WOS:【SCI-EXPANDED(收录号:WOS:001381663400003)】；

基金：We would like to thank Bullet Edits (http://www.bulletedits.cn) for English language editing of the manuscript.

语种：英文

外文关键词：Ampullary carcinoma; Cell line establishment; Xenografted tumor; Short tandem repeat analysis; Drug resistance

摘要：Ampullary carcinoma (AC) of the intestinal type represents a distinct variant within the broader category of ampullary neoplasms. The scarcity of pertinent cellular models has constrained investigations centered on this particular malignancy. This research effectively generated a cell line (CL) of intestinal-type AC (DPC-X3). This newly developed CL has been continuously cultured for 1 year and has demonstrated stable passaging exceeding 60 generations. Morphologically, DPC-X3 exhibited characteristic attributes of an epithelial tumor. The cell proliferation rate of DPC-X3 exhibited a doubling interval of 79 h. Short tandem repeat (STR) analysis validated the high consistency between DPC-X3 and the patient's primary tumor. Characteristically, DPC-X3 displayed sub diploid karyotypes, primarily featuring 44, XY inv (9), -18, -20, -22, and + mar. Under suspension culture conditions, DPC-X3 could efficiently form organoids, and DPC-X3 cells inoculated subcutaneously into NXG mice could form transplanted tumors. Drug susceptibility assays demonstrated that DPC-X3 resisted paclitaxel, oxaliplatin, 5-fluorouracil(5-FU), and gemcitabine. Immunohistochemical (IHC) evaluation revealed affirmative reactivity for CK7 and CK20 within DPC-X3 cells, while CDX2 exhibited no detectable expression. E-cadherin and Vimentin demonstrated positive immunoreactivity, whereas CEA and CA19-9 displayed faint positivity. The Ki-67 proliferation index was determined to be approximately 40%. DPC-X3 presents a valuable experimental platform for elucidating the pathogenesis of intestinal-type AC and can serve as a driver for drug development efforts.