文献类型：期刊文献

英文题名：Comprehensive Pan-Cancer Analysis Reveals the Potential Biological, Immunological, and Prognostic Value of NKG2A

作者：Rong, Yao[1,2,3,4];Wang, Yongfeng[2,3,4,5];Tang, Mingzheng[1,2,3,4];Zhang, Guiqian[1,2,3,4];Yuan, Yuan[6];Dong, Fengyuan[7];Wu, Zhihang[1];Ma, Guorong[1];Liu, Songhua[1,2];Zhao, Xiashuang[1,2,3,4];Cai, Hui[2,3,4,5]

第一作者：Rong, Yao

通信作者：Cai, H[1];Cai, H[2];Cai, H[3];Cai, H[4]

机构：[1]Gansu Univ Chinese Med, Gansu Prov Hosp, Clin Med Coll 1, Lanzhou, Peoples R China;[2]Gansu Prov Hosp, Gen Surg Clin Med Ctr, Lanzhou, Peoples R China;[3]Gansu Prov Hosp, Key Lab Mol Diagnost & Precis Med Surg Oncol Gansu, Lanzhou, Gansu, Peoples R China;[4]Gansu Prov Hosp, NHC Key Lab Diag & Therapy Gastrointestinal Tumour, Lanzhou, Peoples R China;[5]Lanzhou Univ, Clin Med Coll 1, Lanzhou, Peoples R China;[6]Gansu Prov Hosp, Dept Crit Care Med, Lanzhou, Peoples R China;[7]Lianyungang First Peoples Hosp, Lianyugang, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Prov Hosp, Gen Surg Clin Med Ctr, Lanzhou, Peoples R China;[2]corresponding author), Gansu Prov Hosp, Key Lab Mol Diagnost & Precis Med Surg Oncol Gansu, Lanzhou, Gansu, Peoples R China;[3]corresponding author), Gansu Prov Hosp, NHC Key Lab Diag & Therapy Gastrointestinal Tumour, Lanzhou, Peoples R China;[4]corresponding author), Lanzhou Univ, Clin Med Coll 1, Lanzhou, Peoples R China.

年份：2023

卷号：2023

外文期刊名：ANALYTICAL CELLULAR PATHOLOGY

收录：;Scopus(收录号：2-s2.0-85176272995);WOS:【SCI-EXPANDED(收录号:WOS:001088929300001)】；

基金：We would like to thank Key Laboratory of Molecular Diagnostics and Precision Medicine for Surgical Oncology in Gansu Province, and General Surgery Clinical Medical Center of Gansu Provincial Hospital for their contributions. This work was funded by the 2021 Central-Guided Local Science and Technology Development Fund (ZYYDDFFZZJ-1), Gansu Da Vinci robot high-end diagnosis and treatment team construction project, COVID-19 prevention and control technology research project (2020-XG-1), Natural Science Foundation of Gansu Province (Nos. 22JR11RA257, 22JR5RA692, 21JR7RA633 and 21JR1RA038), Gansu University of Chinese Medicine 2023 Postgraduate Innovation and Entrepreneurship Fund Project No. 38, Key Research and Development Plan of Gansu Province (No. 21YF5FA169), Excellent master/doctoral program of Gansu Provincial People's Hospital (22GSSYD-1/67), Gansu Province Excellent Doctor Fund Project (23JRRA1320), and Research project of Traditional Chinese Medicine of Gansu province (GZKZ-2022-6).

语种：英文

摘要：Background. NKG2A (KLRC1) belongs to the NKG2 family, which has been shown to affect the activity of natural killer (NK) cells and CD8T cells. However, a comprehensive biological analysis and exploration of NKG2A in different cancers is lacking and this needs to be further investigated. Methods. A comprehensive pan-cancer analysis of NKG2A was performed based on multiple databases. The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) databases were used to analyze the expression profile of NKG2A in pan-cancer. The relevance of NKG2A to the prognosis of different cancers was assessed using Kaplan-Meier survival analysis. In addition, we explored the correlation between NKG2A expression and gene mutations, pathological staging, tumor-infiltrating immune cells (TIICs), DNA methyltransferase (DNMT) genes, tumor mutation burden (TMB), microsatellite instability (MSI), mismatch repair (MMR), and immune checkpoints (ICPs). Finally, the expression levels of NKG2A in several cancer cell lines were verified by qRT-PCR. Results. Pan-cancer comprehensive analysis showed that NKG2A expression levels were significantly different between multiple cancers and corresponding normal tissues. The differential expression of NKG2A was related to the prognosis and pathological staging of patients with multiple cancers, and was closely related to the excessive infiltration of immune cells and the regulation of ICP genes in the tumor microenvironment (TME). In addition, TMB, MSI, MMR, and DNMT genes in many cancer types are also affected by NKG2A expression. Gene set enrichment analysis (GSEA) showed that NKG2A was associated with multiple immune-related functions and pathways in malignant tumors. qRT-PCR results showed that NKG2A was underexpressed in liver, gastric, and colon cancer cell lines compared to normal cells, which was consistent with bioinformatics analysis. Conclusion. The present study suggests that NKG2A may be a potential predictive biomarker for cancer immune response and prognosis.