文献类型：期刊文献

中文题名：响应面法优化复合酶提取枸杞子总黄酮的工艺研究

英文题名：Optimization of Compound Enzyme Extraction Technology of Total Flavonoids from Lycii Fructus by Response Surface Method

作者：高建德[1];朱晓玉[1];刘雄[1];陈晖[1];宋开蓉[1];师婷婷[1]

第一作者：高建德

机构：[1]甘肃中医药大学药学院,甘肃兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2017

卷号：24

期号：7

起止页码：66

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2017_2018】；

基金：甘肃省科技攻关项目(GYC13-05);甘肃省高等学校科学研究项目(2015B-073)

语种：中文

中文关键词：响应面法;工艺优化;复合酶提取;枸杞子总黄酮

外文关键词：response surface method; technology optimization; compound enzyme extraction; total flavonoids from Lycii Fructus

摘要：目的采用响应面法优化复合酶提取枸杞子总黄酮的工艺条件。方法在单因素试验基础上,选择酶用量、酶解时间、酶解温度为因素,进行3因素3水平的中心组合设计,采用响应面法分析3个因素对枸杞子总黄酮得率的影响。结果复合酶辅助提取枸杞子总黄酮的工艺路线为:复合酶用量0.30%,酶解时间1.0 h,酶解温度60℃。总黄酮得率预测值为0.949 2%。结论响应面法优化复合酶辅助提取枸杞子总黄酮的方法稳定可行,操作简单,可为深入研究及应用提供依据。
Objective To optimize the extraction technology of total flavonoids from Lycii Fructus by response surface methodology （RSM）. Methods On the basis of single-factor tests, enzyme concentration, enzymolysis time, and enzyme solution temperature were selected as influencing factors during extraction to conduct three-factor three-level center combination design. The effects of three factors on the yield of total flavonoids from Lycii Fructus were analyzed by RSM. Results The optimum conditions for extraction technology of total flavonoids from Lycii Fructus were enzyme concentration 0.30%, enzymolysis time 1.0 h, enzyme solution temperature 60 ℃. The predicted extraction yield of total flavonoids was 0.949 2%. Conclusion Using RSM to optimize the extraction technology of total flavonoids from Lycii Fructus is stable, feasible, and simple, which can provide references for further study and application.