文献类型：期刊文献

中文题名：基于NF-κB/COX-2通路探讨祛瘀温经汤治疗寒凝血瘀型原发性痛经的作用机制

英文题名：Investigating the mechanism of Quyu Wenjing Decoction in alleviating primary dysmenorrhea with cold coagulation and blood stasis via the NF-κB/COX-2 pathway

作者：李新梅[1];王蓉[1];水蓉枝[1];张怡[1];王新斌[1]

第一作者：李新梅

机构：[1]甘肃中医药大学中西医结合学院,甘肃兰州730000

第一机构：甘肃中医药大学中西医结合学院

年份：2025

卷号：36

期号：5

起止页码：838

中文期刊名：时珍国医国药

外文期刊名：Lishizhen Medicine and Materia Medica Research

收录：;北大核心:【北大核心2023】；

基金：2023年甘肃省自然科学基金(23JRRA1206);甘肃省高校教师创新基金项目(2023B-109)。

语种：中文

中文关键词：原发性痛经;祛瘀温经汤;核转录因子-kappaB;环氧化酶-2

外文关键词：Primary dysmenorrhea;Quyu Wenjing Decoction;NF-κB;COX-2

摘要：目的基于核转录因子-kappaB(NF-κB)/环氧化酶-2(COX-2)通路探讨祛瘀温经汤对寒凝血瘀型原发性痛经大鼠干预作用及机制。方法将60只雌性SD大鼠随机分为空白组、模型组、祛瘀温经汤低、中、高剂量组、少腹逐瘀颗粒组,药物干预后观察大鼠扭体反应、扭体反应潜伏期;检测血液流变学;苏木素-伊红(HE)染色观察子宫组织病理形态;酶联免疫吸附测定法(ELISA)法检测相关细胞因子水平;蛋白免疫印迹(Westren Blot)法检测NF-κB、COX-2表达;实时荧光定量聚合酶链式反应(Real-time PCR)法检测NF-κB、COX-2 mRNA表达。结果与空白组相比,模型组大鼠出现扭体反应,子宫组织大量炎症细胞浸润,IL-6、TNF-α、PGF2α含量升高,PGE2含量降低,NF-κB、COX-2表达及mRNA水平升高(P<0.05);与模型组相比,治疗组大鼠扭体潜伏时间延长,扭体反应次数减少,子宫组织炎症浸润状态改善,IL-6、TNF-α、PGF2α含量降低,PGE2含量升高,NF-κB、COX-2表达及mRNA水平降低(P<0.05)。结论祛瘀温经汤可能通过调节NF-κB/COX-2通路,抑制炎症相关蛋白表达,修复子宫病理损伤,有效缓解寒凝血瘀型原发性痛经大鼠疼痛症状。
Objective To investigate the therapeutic effects and underlying mechanism of Quyu Wenjing Decoction(QYWJD)on primary dysmenorrhea(PD)with cold coagulation and blood stasis in rats based on the nuclear factor-kappa B(NF-κB)/cyclooxygenase-2(COX-2)signaling pathway.Methods Sixty female Sprague-Dawley(SD)rats were randomly assigned to the following groups:the blank group,model group,the QYWJD low-,medium-,and high-dose groups,and the Shaofu Zhuyu Granule(SFZYG)group.After the drug intervention,the writhing response and its latency were observed.Hemorheological parameters were measured,and hematoxylin-eosin(HE)staining was performed to observe uterine tissue pathology.Cytokine levels were determined using enzyme-linked immunosorbent assay(ELISA),while NF-κB and COX-2 protein expression levels were assessed by Western blot(WB).The mRNA expression levels of NF-κB and COX-2 were detected via real-time PCR.Results Compared with the blank group,rats in the model group exhibited significant writhing responses,increased infiltration of inflammatory cells in uterine tissue,elevated levels of IL-6,TNF-α,and PGF2α,reduced PGE2 levels,and upregulated NF-κB and COX-2 protein and mRNA expression(P<0.05).Compared with the model group,the QYWJD-treated groups demonstrated prolonged latency of writhing response,fewer writhing episodes,reduced inflammatory cell infiltration in the uterine tissues,decreased IL-6,TNF-α,and PGF2α levels,increased PGE2 levels,and reduced NF-κB and COX-2 and mRNA expression(P<0.05).Conclusion QYWJD may alleviate PD with cold coagulation and blood stasis by modulating the NF-κB/COX-2 pathway,inhibiting the expression of inflammation-related proteins,and repairing the uterine tissue damage.