文献类型：期刊文献

中文题名：黄芪注射液对宫颈永生化上皮细胞生长的作用及其细胞周期调控机理

英文题名：Effect of Astragalus injection on cervical immortalized epithelial cell growth and its cell cycle regulation mechanism

作者：吕玲[1];肖晨光[2];张立[3];金莉娅[1];刘青[1];代志蓉[1]

第一作者：吕玲

机构：[1]甘肃省妇幼保健院国际医疗部LDR区,甘肃兰州730050;[2]中核兰州504医院,甘肃兰州730065;[3]甘肃中医学院,甘肃兰州730000

第一机构：甘肃省妇幼保健院国际医疗部LDR区,甘肃兰州730050

年份：2016

卷号：22

期号：4

起止页码：328

中文期刊名：海南医学院学报

外文期刊名：Journal of Hainan Medical University

收录：CSTPCD

基金：甘肃省中医药管理局课题(GZK-2013-56)~~

语种：中文

中文关键词：宫颈癌;黄芪注射液;宫颈上皮内瘤变;细胞周期

外文关键词：Cervical cancer; Cervical intraepithelial neoplasia; Astragalus injection; Cell cycle

摘要：目的:研究黄芪注射液对宫颈永生化上皮细胞生长的调节作用及细胞周期调控机理。方法:选择宫颈永生化上皮细胞(H8细胞)进行实验,用不同浓度的黄芪注射液进行处理,采用MTT法检测细胞活力,流式细胞术检测不同细胞周期的比例以及不同免疫细胞亚群的含量,采用PCR法检测细胞周期相关蛋白的mRNA含量。结果:黄芪注射液处理能够以剂量依赖性的方式降低细胞活力,200μg/mL浓度的抑制效应最显著;200μg/mL黄芪处理组的NK细胞、CD3^+CD4^+CD8^-T细胞含量以及CD3^+CD4^+CD8^-/CD3^+CD4^-CD8^+T细胞比例高于0μg/mL黄芪处理组,CD3^+CD4^-CD8^+T细胞含量低于0μg/mL黄芪处理组;200μg/mL黄芪处理组的G0/G1期、S期细胞比例以及cyclinA、cyclinB、cyclinD1的mRNA含量低于0μg/mL黄芪处理组,G2/M期细胞比例高于0μg/mL黄芪处理组(均P<0.05)。结论:黄芪注射液对宫颈永生化上皮细胞的生长具有抑制效应,其作用途径包括调节免疫细胞含量、停滞细胞周期和下调细胞周期相关蛋白的表达。
Objective： To study the regulating effect of Astragalus injection on cervical immortalized epithelial cell growth and cell cycle regulation mechanism. Methods： Cervical immortalized epithelial cells （H8 cells） were selected for experiment and processed with different concentrations of Astragalus injection. MTT method was used to detect cell viability, flow cytometry was used to detect the percentages of different cell cycles and the contents of different immune cell subsets, and PCR method was used to detect mRNA contents of cell cycle-related proteins. Results= Astragalus injection could reduce cell viabili- ty in a dose-dependent manner, and the inhibiting effect of 200/＊g/mL concentration was the most significant. NK cell and CD3 ＋CD4--CDS-T cell contents as well as CD3＋CD4＋CDS-/CD3＋CD4-CD8＋-T cell percentage IN 200 t^g/mL Astragalus pro- cessing group was higher than those of 0 ug/mL Astragalus-processing group, and CD3 q-CD4-CDS@T cell content was lower than that of 0 ug/mL Astragalus-processing group. Percentages of G0/G1 phase and S phase cells as well as mRNA contents of cyclinA, cyclinB and cyclinD1 of 200 t^g/mL Astragalus-processing group were lower than those of 0 t^g/mL Astragalus-pro eessing group, and percentage of G2/M phase cell was higher than that of 0/~g/mL Astragalus-processing group. Conclusions= Astragalus injection has inhibiting effect on the growth of cervical immortalized epithelial cells, and its mechanisms include reg ulating immune cell contents, arresting cell cycle and down-regulating cell cycle-related protein expression.