文献类型：期刊文献

中文题名：离子辐射选育当归新品种安全性评价

英文题名：Safety evaluation of new Angelica sinensis varieties cultivated with ionizing radiation

作者：李硕[1,2,3];李成义[1,2,3];李敏[4];杨秀娟[1,3];冯新昌[1,3]

第一作者：李硕

机构：[1]甘肃中医药大学,甘肃兰州730000;[2]甘肃省高校中(藏)药化学与质量研究省级重点实验室,甘肃兰州730000;[3]甘肃省中药质量与标准研究重点实验室培育基地,甘肃兰州730000;[4]成都中医药大学,四川成都610075

第一机构：甘肃中医药大学

年份：2018

卷号：49

期号：11

起止页码：2662

中文期刊名：中草药

外文期刊名：Chinese Traditional and Herbal Drugs

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：国家自然科学基金资助项目(81360622);甘肃省中医药管理局项目(GZK-2017-7);甘肃省高等学校科学研究项目(2016B-055);甘肃省自然科学基金项目(1508KJ2A013)

语种：中文

中文关键词：辐射选育;当归;新品种;安全性评价;灰色关联

外文关键词：irradiation breeding;Angelica sinensis （Oliv.） Diels;new species;safety evaluation;grey correlation analysis

摘要：目的对离子辐射选育当归新品种进行安全性评价。方法采用气相色谱法测定有机氯类农药残留量;电感耦合等离子体质谱法测定Pb、Cd、Cu含量;测汞仪测定Hg含量;原子荧光法测定As含量。以总灰分、酸不溶性灰分、重金属及有害元素含量为指标,利用灰色关联度法,结合小鼠急性毒性实验,对样品安全性进行综合评价。结果辐射选育当归新品种岷归3号、岷归4号药材中有机氯类农药残留量、重金属和有害元素含量符合《中华人民共和国药用植物及制剂进出口绿色行业标准》规定。灰色关联度法对样品安全性进行综合评价表明,岷归3号、岷归4号整体安全性优于其他样品。当归不同部位对重金属及有害元素的富集程度不同,归头对重金属Pb、Cd、Cu的富集程度较高,归尾对重金属As、Hg的富集程度较高,归身对重金属的富集程度相对较低。小鼠急性毒性实验未测得ig给药小鼠半数致死量(LD50),而小鼠对其最大耐受量为120 g/kg,相当于临床成人(60 kg)最大用量的480倍。结论当归辐射选育品种药材无明显毒性,临床常用量安全可行。
Objective To evaluate the safety of new Angelica sinensis species with ionizing radiation. Methods GC was adopted to determine the organochlorine pesticide residues; ICP-MS was used to identify the contents of lead（Pb）, cadmium（Cd）, and copper（Cu）; Mercury vapourmeter was used to determine the content of mercury（Hg）; Atomic fluorescence spectrometry was used to identify the content of asenic（As）. Adopting gray correlation as experiment method and taking the total ash, acid-insoluble ash, heavy metals, and harmful elements content as evaluation indexes, combined with acute toxicity test on mice, the study is to evaluate the safety of the new A. sinensis varieties cultivated with ionizing radiation. Results The results show that the organochlorine pesticide residues, heavy metals, and harmful elements contents in Mingui No.3 and Mingui No.4 comply with regulations in ＂Green Industry Standards for the Import and Export of Medicinal Plants and Preparations of the People＇s Republic of China＂. Comprehensive evaluation of the safety of the samples with gray correlation degree showed that the safety of Mingui No.3 and Mingui No.4 was superior to other samples. The enrichment degrees of different parts of A. sinensis on heavy metals and harmful elements were different： The head of A. sinensispresented higher enrichment degree on the heavy metals lead（Pb）, cadmium（Cd）, and copper（Cu）, the tail shows higher enrichment degree on such heavy metals as arsenic（As） and mercury（Hg）, and the body presented lower enrichment degree on heavy metals. In the acute toxicity experiment of mice, LD50 was not measured in the mice and the maximum tolerance of mice was 120 g/kg, equivalent to 480 times the clinically maximum amount of adults（60 kg）. Conclusion There is no obvious toxicity in new A. sinensis species with ionizing radiation, and the clinical usual dosage is safe and feasible.