文献类型：期刊文献

英文题名：In vitro Immunomodulatory Activity of Oxymatrine on Toll-Like Receptor 9 Signal Pathway in Chronic Hepatitis B

作者：Yao, Ning[1];Wang, Xin[2]

第一作者：姚凝

通信作者：Wang, X[1]

机构：[1]Gansu Univ Tradit Chinese Med, Clin Coll Med, Lanzhou 730000, Gansu, Peoples R China;[2]Xi An Jiao Tong Univ, Dept Infect Dis, Xian 710061, Shaanxi, Peoples R China

第一机构：甘肃中医药大学临床医学院

通信机构：[1]corresponding author), Xi An Jiao Tong Univ, Dept Infect Dis, Xian 710061, Shaanxi, Peoples R China.

年份：2014

卷号：42

期号：6

起止页码：1399

外文期刊名：AMERICAN JOURNAL OF CHINESE MEDICINE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000347026800008)】；

基金：This work was supported by the Natural Science Foundation of Gansu Province, China (Grant No. 0710RJZA010) and Zhengzhou Municipal Natural Science Foundation (Grant No. 201303231). Editorial revision of this manuscript was provided in part by: Tom D.Y. Chin, MD, MPH, University of Kansas Medical Center, Kansas City, Kansas, USA.

语种：英文

外文关键词：Oxymatrine; Toll-Like Receptor; Immunomodulatory Activity; Hepatitis B Virus

摘要：Oxymatrine, extracted from the herb Sophora alopecuraides L., was investigated to determine its anti-HBV immunomodulatory mechanism in vitro. Human peripheral lymphocytes were isolated from heparinized whole blood from 48 chronic hepatitis B (CHB) patients. The lymphocytes from each patient were divided into two groups according to pretreatment or no pretreatment with Oxymatrine in vitro. We examined the changes of expression and function of the toll-like receptor 9 (TLR9) signal transduction pathway in the peripheral lymphocytes with different treatment methods and investigated the synergism of Oxymatrine and the TLR9 ligand on antiviral cytokine secretions in vitro. The data showed Oxymatrine could induce antiviral cytokine secretions directly from the peripheral lymphocytes. For the TLR9 signal pathway, Oxymatrine not only augmented the expressions of TLR9 signal transduction molecules, but also activated the TLR9 signal function. This study has clearly demonstrated that TLR9 ligand could stimulate peripheral lymphocytes that have been pretreated with Oxymatrine. Furthermore, the quantity of antiviral cytokines secreted by the pretreated lymphocytes was greater than that of those without pretreatment. The interaction between the Oxymatrine and the TLR9 ligand appears to be synergistic. This study suggests Oxymatrine could be a strong immunomodulator, influence TLR9 signaling transduction, and synergistically improve the immune efficacy of the TLR9 ligand against CHB.