详细信息

当归、黄芪注射液对鸡胚绒毛尿囊膜模型的毒性研究     被引量:4

Study on Toxicity of Angelica Sinensis Injection and Astragalus Membranaceus Injection in Chorioallantoic Membrane Model

文献类型:期刊文献

中文题名:当归、黄芪注射液对鸡胚绒毛尿囊膜模型的毒性研究

英文题名:Study on Toxicity of Angelica Sinensis Injection and Astragalus Membranaceus Injection in Chorioallantoic Membrane Model

作者:杨丽霞[1];豆伍应[2];颜春鲁[3];刘铜华[1];魏道武[3]

第一作者:杨丽霞

机构:[1]北京中医药大学,北京100029;[2]兰州市榆中县第一人民医院,兰州榆中730100;[3]甘肃中医学院,甘肃兰州730000

第一机构:北京中医药大学,北京100029

年份:2009

卷号:15

期号:5

起止页码:46

中文期刊名:中国实验方剂学杂志

外文期刊名:Chinese Journal of Experimental Traditional Medical Formulae

收录:CSTPCD;;北大核心:【北大核心2008】;

基金:高等学校学科创新引智计划资助(B07007)

语种:中文

中文关键词:当归注射液;黄芪注射液;绒毛尿囊膜;毒性

外文关键词:ASI-angelica sinensis injection; AMI-astragalus membranaceus injection; CAM-chorioallantoic membrane assay ; toxicity

摘要:目的:通过观察当归、黄芪注射液对鸡胚绒毛尿囊膜(CAM)模型的毒性,探讨当归、黄芪注射液利用CAM模型研究血管生成的可行性。方法:制备CAM模型,观察应用当归、黄芪注射液及不同配比后CAM模型的存活情况。结果:当归、黄芪注射液及不同配比对CAM模型的存活无毒性,与生理盐水对照组相比无显著性差异(P>0.05)。结论:可以利用CAM模型研究当归、黄芪注射液的促血管生成作用。
Objective:To explore the toxicity of Angelica sinensis injection (ASI) and Astragalus membranaceus injection (AMI) in the chick embryo chorioallantoic membrane (CAM) model. Methods: Fertilized eggs were incubated for 7 days at 37.8℃, and randomly divided into 6 groups, and then were respectively treated with NS (negative control), ASI, AMI, and different ratio of ASI and AMI. After another 3 days of incubation, the number of living chick embryos were counted. Results:The survival of chick embryos could not be inhibited by ASI and AMI, and our assay showed that no difference between therapy groups and negative control ( P 〉 0.05). Conclusion: ASI and AMI could be used to study angiogenesis by CAM.

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