文献类型：期刊文献

中文题名：不同X线剂量照射对骨髓间充质干细胞的增殖及DNA损伤的影响

英文题名：Effects of different doses of X-ray irradiation on the proliferation and DNA damage of bone marrow mesenchymal stem cell

作者：周妮娜[1];张利英[1];刘永琦[2];卢志伟[1];丁楠[3];刘涌爱[3];华君瑞[3];武有明[1]

第一作者：周妮娜

机构：[1]甘肃中医药大学/甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室,兰州730000;[2]敦煌医学与转化省部共建教育部重点实验室,兰州730000;[3]甘肃省空间辐射生物学重点实验室,中国科学院近代物理研究所,兰州730000

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2016

卷号：29

期号：9

起止页码：923

中文期刊名：医学研究生学报

外文期刊名：Journal of Medical Postgraduates

收录：CSTPCD;;北大核心:【北大核心2014】；

基金：国家自然科学基金(81473457)

语种：中文

中文关键词：辐射;骨髓间充质干细胞;DNA损伤;基因组不稳定性

外文关键词：Radiation; Bone marrow mesenchymal stem cell; DNA damage; Genomic instability

摘要：目的低剂量电离辐射对骨髓间充质干细胞(human mesenchymlstem cells-bone marrow,HMSC-bm)有兴奋效应,但高剂量电离辐射对该细胞的损伤作用少见报道。文中旨在研究不同X线剂量照射对体外骨髓间充质干细胞细胞增殖及DNA损伤影响。方法采用人HMSC-bm体外进行培养,实验分为对照组及照射组,以0、0.5、1、2、4、6、8 Gy不同X射线分别照射细胞,采用CCK8法检测细胞增殖水平;以0、1、2、4 Gy不同剂量照射细胞:CB法检测各组细胞微核率;免疫荧光检测照射后0.5、2、12、24 h不同时间点53BP1免疫荧光簇焦点(foci)数目的变化。结果随辐照剂量的增加,与0 Gy比较,除第3天0.5、1 Gy外,同一时间各组细胞吸光度值(A值)逐渐减少(P<0.05)。与同一辐照剂量第1天比较,0.5、1、4 Gy第3、4、5天A值增加(P<0.05),0、6 Gy第4、5天A值增加(P<0.05),2、8 Gy第5天A值增加(P<0.05)。细胞受照后0.5 h均出现foci点,达到最高峰,后随着时间的延长,foci点逐渐减少;不同剂量同一时间相比,随着剂量的增加,各个时间点免疫荧光簇集点增加,同一剂量不同时间相比,随着时间的增加免疫荧光焦点逐渐减少(P<0.05)。与对照组细胞微核率[(6.333±2.031)%]相比,随着辐射剂量增加(1、2、4 Gy)细胞微核率逐步上升[(23.667±1.582)%、(42.000±4.583)%、(777.333±2.082)%],差异有统计学意义(P<0.05)。结论 HMSC-bm对高剂量X射线具有敏感性,基因组不稳定,其自我修复能力受到剂量的影响,在运用放射疗法及临床治疗上应该加强其应用安全性。
Objective Low-dose ionizing radiation has an excitatory effect on human mesenehymal stem cells-bone marrow （ HMSCs-bm）, but reports are rarely seen on high-dose ionizing radiation-induced damage to HMSCs-bm. This study was to investigate the effects of X-ray irradiation on the proliferation and DNA damage HM- SCs-bm. Methods HMSCs-bm were cultured and exposed to 0, O. 5, 1, 2, 4, 6, and 8 Gy X-ray irradiation, respectively. Then the proliferation of the cells was detected using the CCK8 method. The CB method was employed to measure the micronncleus rate of the ceils exposed to 0, 1, 2, and 4 Gy X-ray irradiation, and immunofluoreseence adopted to observe the changes in the focus （foci） of 53BP1 at 0.5, 2, 12, and 24 h after exposure. Results The absorbance value of different groups of cells was gradually decreased with the increasing dose of irradiation at the same time point （P 〈 0. 05 ）, except at 0.5 and 1 Gy on the 3rd day. Compared with the 1 st day, the absorbance value was significantly increased at 0.5, 1, and 4 Gy on the 3rd, 4th, and 5th day （P 〈0.05） as well as at 0 and 6 Gy on the 2nd, 4th, 5th, and 8th day （P 〈0.05）. The 53BP1 foei began to appear at 0.5 h after exposure to irradiation, increasing in number in a dose-dependent manner, reaching the peak, and then gradually reduced. The rate micronuclei in the cells was increased with the increased dose of radiation, （23. 667 ± 1.582）% at 1 Gy, （42.000±4.583）% at2 Gy, and （777.333 ±2.082）% at4 Gy, as compared with （6.333 ±2.031）% at0 Gy, （P 〈 0.05）. Conclusion HMSCs-bm are sensitive to the high-dose X-ray irradiation and has a genomic instability. Their self- repair ability is affected by the dose of irradiation and therefore safety should be stressed in the clinical application of radiation therapy and anti-radiation measures.