文献类型：期刊文献

中文题名：甘草及其内生菌通过调节肠道菌群和Th17/Treg细胞平衡改善三硝基苯磺酸诱导的溃疡性结肠炎

英文题名：Glycyrrhizae Radix et Rhizoma and its endophytes ameliorate trinitrobenzene sulfonic acid-induced ulcerative colitis by modulating intestinal flora and Th17/Treg cell balance

作者：高广淼[1];杨玲玲[1];杨秀娟[1];付晓艳[1];李洁[1];马趣环[2];连小龙[3];杨志军[1];邓毅[1]

第一作者：高广淼

机构：[1]甘肃中医药大学,甘肃兰州730000;[2]甘肃省医学科学研究院,甘肃兰州730000;[3]青海大学,青海西宁810016

第一机构：甘肃中医药大学

年份：2025

卷号：50

期号：21

起止页码：6165

中文期刊名：中国中药杂志

外文期刊名：China Journal of Chinese Materia Medica

收录：;北大核心:【北大核心2023】；

基金：国家自然科学基金项目(81960723);甘肃省自然科学基金项目(21JR11RA145,22JR11RA112);甘肃省优秀博士生项目(22JR5RA577);兰州市科技计划项目(2022-3-21);甘肃省中医药管理局项目(GZKP-2022-37)。

语种：中文

中文关键词：甘草;内生菌;溃疡性结肠炎;肠道菌群;有效成分

外文关键词：Glycyrrhizae Radix et Rhizoma;endophytes;ulcerative colitis;intestinal flora;active ingredient

摘要：溃疡性结肠炎(UC)是一种慢性复发性肠道炎症疾病,主要累及结肠黏膜。临床上用于治疗UC的药物常伴有较为严重的不良反应。甘草作为我国传统中药,具有抗炎、调节免疫及抗氧化损伤等药理作用,对治疗UC十分有益。该研究建立了UC大鼠模型,探究甘草及内生菌通过调节肠道菌群和辅助性T细胞17(Th17)/调节性T细胞(Treg)平衡治疗UC的作用机制。选用无特定病原体(SPF级)SD大鼠64只,除空白组外,其余使用三硝基苯磺酸(TNBS)诱导建立UC大鼠模型成功后,随机分为模型组,柳氮磺胺吡啶组,甘草醇提取物(LAE)高、中、低剂量组,鼠李糖乳杆菌组,甘草内生菌(JTZB55)组,分别给予相应药物。根据一般情况、疾病活动指数(DAI)评分、结肠长度、体质量、苏木素-伊红(HE)染色结肠组织病理切片,评价甘草及内生菌的治疗效果;流式细胞术检测Th17/Treg细胞凋亡;逆转录-聚合酶链式反应(RT-PCR)检测维甲酸相关孤儿受体(ROR-γt)、叉头框蛋白(Foxp3)表达水平;采用16S rRNA技术检测肠道菌群变化。结果显示,甘草及其内生菌能够有效缓解TNBS诱导的结肠炎恶化。其中,LAE及JTZB55可通过调节细胞因子[白细胞介素(IL)-6、IL-17A、IL-10],发挥免疫调节作用,使TNBS诱导失衡的Th17/Treg细胞恢复平衡。16S rRNA检测显示,经LAE及JTZB55处理后,UC大鼠肠道菌群结构得到明显改善。综上所述,LAE及JTZB55具有抑制UC大鼠炎症,调节Th17/Treg细胞平衡以及改善肠道菌群的作用,能够有效缓解TNBS诱导的UC。
Ulcerative colitis(UC)is a chronic relapsing inflammatory disease of the intestines that primarily involves the colonic mucosa.More serious adverse reactions often accompany drugs used clinically for the treatment of UC.Glycyrrhizae Radix et Rhizoma,as a traditional Chinese medicine in China,has pharmacological effects such as anti-inflammatory,immune-regulating and antioxidant damage,which are very beneficial for the treatment of UC.In this study,we investigated the mechanism of action of Glycyrrhizae Radix et Rhizoma and its endophytes in treating UC by regulating the intestinal flora and the balance of T-cell 17(Th17)/regulatory T-cells(Treg)by establishing a UC rat model.Sixty-four specific pathogen free(SPF)SD rats were selected to establish UC rat model using trinitrobenzene sulfonic acid(TNBS)except for blank group,which was then randomly divided into model group,salicylazosulfapyridine group,licorice alcohol extract(LAE)-high,-medium,and-low dosage groups,lactobacillus rhamnosus group,and licorice endophyte(JTZB55)group,were given the appropriate drugs.The therapeutic effects of licorice and its endophytes were evaluated based on general condition,disease activity index(DAI)score,colon length,body weight,and hematoxylin-eosin(HE)stainning colon histopathological sections.Flow cytometry was used to detect Th17/Treg cell apoptosis.Reverse transcription-polymerase chain reaction(RT-PCR)was used to detect the expression levels of retinoic acid-related orphan receptor(ROR-γt)and forkhead frameshift protein(Foxp3).And changes in intestinal flora were detected using 16S rRNA technology.The results showed that Glycyrrhizae Radix et Rhizoma and its endophytes could effectively alleviate the deterioration of TNBS-induced colitis.Among them,LAE and JTZB55 could play an immunomodulatory role by regulating cytokines[interleukin(IL)-6,IL-17A,IL-10]to restore the balance of TNBS-induced imbalance of Th17/Treg cells.16S rRNA assay showed that the intestinal flora structure of the UC rats after the treatment with LAE and JTZB55 was significantly improved.In conclusion,LAE and JTZB55 can inhibit inflammation,regulate the balance of Th17/Treg cells and improve the intestinal flora in UC rats,which can effectively alleviate TNBS-induced UC.