文献类型：期刊文献

英文题名：Study on the signaling pathways involved in the anti-hyperglycemic effect of raspberry ketone on zebrafish using integrative transcriptome and metabolome analyses

作者：Zhu, Xinliang[1,3];Zhang, Dengcai[4];Wang, Yong[5];Wang, Chuangxin[1];Liu, Xiaoxiao[6];Niu, Yicong[2]

第一作者：Zhu, Xinliang

通信作者：Zhu, XL[1];Niu, YC[2];Zhu, XL[3]

机构：[1]Northwest Normal Univ, Coll Life Sci, Lanzhou 730070, Gansu, Peoples R China;[2]Lanzhou Univ, Hosp 1, Dept Gen Surg, Lanzhou 730000, Gansu, Peoples R China;[3]Gansu Prov Hosp, Dept Sci Res, Lanzhou 730000, Gansu, Peoples R China;[4]Gansu Prov Matern & Hlth Care Hosp, Dept Pathol, Lanzhou 730000, Gansu, Peoples R China;[5]Gansu Univ Chinese Med, Pathol Ctr, Lanzhou 730000, Gansu, Peoples R China;[6]Lanzhou Inst Food & Drug Control, Lanzhou, Gansu, Peoples R China

第一机构：Northwest Normal Univ, Coll Life Sci, Lanzhou 730070, Gansu, Peoples R China

通信机构：[1]corresponding author), Northwest Normal Univ, Coll Life Sci, Lanzhou 730070, Gansu, Peoples R China;[2]corresponding author), Lanzhou Univ, Hosp 1, Dept Gen Surg, Lanzhou 730000, Gansu, Peoples R China;[3]corresponding author), Gansu Prov Hosp, Dept Sci Res, Lanzhou 730000, Gansu, Peoples R China.

年份：2024

外文期刊名：FOOD & FUNCTION

收录：;EI(收录号：20243516954987);Scopus(收录号：2-s2.0-85202459077);WOS:【SCI-EXPANDED(收录号:WOS:001298431300001)】；

基金：The Project for Promoting the Innovative Capacity of College and University from Gansu Province Education Department (2023A-007), the Natural Science Foundation of Gansu Province (grant no. 21JR7RA563, 21JR7RA636, 23JRRA1483), Lanzhou Guiding Program Project (grant no. 2023-ZD-31).

语种：英文

外文关键词：Gene expression - Glucose - Metabolites

摘要：Hyperglycemia leads to increased oxidative stress in mitochondria, an abnormal activation of intracellular inflammatory signals, and mediate multiple dysfunctions. Raspberry ketone (RK) is an aromatic phenolic compound found in many plants and could contribute to weight loss, restore impaired glucose tolerance, and has antioxidant properties. In our investigation, RK could greatly prevent islet, brain and other tissue damage caused by hyperglycemia in a zebrafish model with streptozotocin (STZ)-induced hyperglycemia. Body weight, insulin level, and food intake indexes were also restored by RK. Using transcriptome profiling, we found that RK administration could significantly attenuate STZ-induced insulin synthesis and pancreatic secretion as well as alter protein and carbohydrate metabolism. Metabolomics analysis results showed that RK could also prevent STZ-induced metabolic disorders, such as adenosine and sphingolipid metabolism. Integrative analysis of metabolome and transcriptome data and qRT-PCR validation of key metabolic regulatory genes (glut1, glut2, ctrb1, ccka, gck, pklr) confirmed that the purine pathway was the most enriched metabolic pathway, in which both metabolite accumulation and gene expression levels showed consistent change patterns upon RK treatment. Our study provides a new perspective for understanding the hypoglycemic mechanism of RK and may be helpful for investigating the modes of action of hypoglycemic drugs using the zebrafish hyperglycemia model. Raspberry ketone alleviated hyperglycemia via regulating insulin synthesis and secretion pathways and homeostasis of purine metabolism in a zebrafish hyperglycemia model.