文献类型：期刊文献

中文题名：黄芪多糖对低氧环境中BMSCs成骨分化的影响

英文题名：Effects of Astragalus polysaccharides on Osteogenic Differentiation of BMSCs Cultured in Low Oxygen Environment

作者：伍志伟[1,2];刘丹[1];薛娜[1];刘永琦[1,2];苏韫[1];颜春鲁[1]

第一作者：伍志伟

机构：[1]甘肃中医药大学基础医学院,兰州730000;[2]甘肃省高校重大疾病分子医学与中医药防治省级重点实验室,兰州730000

第一机构：甘肃中医药大学基础医学院（敦煌医学研究所）

年份：2017

卷号：39

期号：6

起止页码：732

中文期刊名：中国细胞生物学学报

外文期刊名：Chinese Journal of Cell Biology

收录：CSTPCD;;CSCD:【CSCD2017_2018】；

基金：甘肃省自然科学基金(批准号:1212RJ2A081);甘肃省省属普通本科高校基本科研业务费专项(批准号:2305136301)资助的课题~~

语种：中文

中文关键词：骨髓间充质干细胞;成骨分化;黄芪多糖;低氧环境

外文关键词：bone mesenchymal stem cells; osteogenic differentiation; Astragalus polysaccharides; low oxygen environment;

摘要：该文研究了黄芪多糖(Astragalus polysaccharides,APS)对低氧环境中成骨诱导的骨髓间充质干细胞(bone mesenchymal stem cells,BMSCs)成骨分化的作用。BMSCs培养于含40μg/mL APS、氧浓度分别为3%、6%、10%和20%的成骨诱导培养液4周,通过茜素红染色分析BMSCs中骨钙蛋白沉积、Real-time PCR和Western blot分别检测成骨相关基因骨髓基质细胞核心结合因子α1(runtrelated transcription factor 2,Runx2)和骨形态发生蛋白-2(bone morphogenetic protein-2,BMP-2)的mRNA和蛋白质水平。结果表明,在不同氧浓度下,与单纯成骨诱导剂组相比,含APS的成骨诱导剂组BMSCs中出现了大量桔红色的骨钙蛋白沉积,Runx2和BMP-2的mRNA和蛋白质水平显著升高;尤其在氧浓度为6%时,差异较显著(P<0.05)。40μg/mL APS具有促进低氧环境中成骨诱导的BMSCs成骨分化的作用,其促进作用与氧浓度相关。
In the present study, the effects of Astragalus polysaccharides（APS） on osteogenic differentiation of bone mesenchymal stem cells（BMSCs） induced by osteogenic medium under hypoxic environment were analyzed. BMSCs were cultured by osteogenic induction medium containing 40 μg/m L Astragalus polysaccharides under the different oxygen concentration（3%, 6%, 10% and 20%） environments for 4 weeks. The osteocalcin particles existing in BMSCs-induced were detected by alizarin red staining. The levels of m RNA and protein of osteoblastic genes, runt-related transcription factor 2（Runx2） and bone morphogenetic protein-2（BMP-2）, in the BMSCs-induced were determined by Real-time PCR and Western blot, respectively. The results showed that, compared with the BMSCs-induced by pure osteogenic induction medium, more red orange osteocalcin particles were observed in BMSCs cultured in the medium containing Astragalus polysaccharide, and the m RNA and protein levels of Runx2 and BMP-2 were significantly increased, especially in 6% oxygen concentration（P〈0.05）. 40 μg/m L Astragalus polysaccharides could promote osteogenic differentiation of BMSCs-induced by osteogenic medium under hypoxic environment, and the role was related to oxygen concentration.