文献类型：期刊文献

中文题名：电针对去卵巢大鼠Wnt3a和β-catenin的基因及蛋白表达的影响

英文题名：Effects of electroacupuncture on mRNA and protein expression of Wnt3a and β-catenin in ovariectomized rats

作者：王亚军[1];张来举[1];浪万英[1];宋亚文[1];宋凯[1]

第一作者：王亚军

机构：[1]甘肃中医药大学针灸推拿学院

第一机构：甘肃中医药大学针灸推拿学院

年份：2019

卷号：25

期号：1

起止页码：6

中文期刊名：中国骨质疏松杂志

外文期刊名：Chinese Journal of Osteoporosis

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：国家自然科学基金地区基金项目(81260584)

语种：中文

中文关键词：骨质疏松症;电针;Wnt3a;β-catenin

外文关键词：osteoporosis;electroacupuncture;Wnt3a;β-catenin

摘要：目的研究电针对去卵巢骨质疏松模型大鼠Wnt3a和β-catenin的蛋白及基因表达的影响。方法选取60只雌性SD大鼠,随机分为空白组、假手术组、模型组、药物组、针刺组,每组12只。空白组不做任何处理,常规饲养;假手术组只暴露双侧卵巢但不切除;其余3组行双侧切卵巢法建立骨质疏松模型大鼠。造模13周后开始治疗,针刺组取"肾俞"、"脾俞"、"三阴交"、"足三里",每次电针刺激15 min(断续波,2 Hz,1.0 mA)并灌服与药物组相同体积的蒸馏水,每日1次,连续治疗5 d,间隔2 d,20 d为1个疗程,共治疗3个疗程;药物组以戊酸雌二醇水溶液(0.02 mg/mL,1 mL/100 g体质量)灌胃,疗程同针刺组;假手术组、模型组灌服与药物组相同体积的蒸馏水,疗程同针刺组;各组大鼠每周称重1次,以此调整给药剂量。治疗结束后麻醉处死大鼠,分离股骨和胫骨,分别用RT-PCR分析法和免疫组化法检测各组大鼠股骨和胫骨Wnt3a,β-catenin蛋白及基因的表达。结果模型组Wnt3a和β-catenin蛋白表达量最低,与空白组和假手术组比较差异有统计学意义(P<0.05);经针刺或药物干预后,Wnt3a和β-catenin蛋白表达显著升高,与模型组比较差异具有统计学意义(P<0.05);模型组Wnt3a和β-catenin mRNA表达量最低,与空白组和假手术组比较差异有统计学意义(P<0.05);经针刺或药物干预后,Wnt3a和β-catenin mRNA表达显著升高,与模型组比较具有统计学意义(P<0.05)。结论针刺防治绝经后骨质疏松症的分子机制可能是通过调控Wnt3a和β-catenin的基因和蛋白表达水平,从而激活Wnt/β-catenin信号通路,以此调节骨重建系统的平衡。
Objective To study the effects of electroacupuncture on gene and protein expression of Wnt3a and β-catenin in ovariectomized rats.Methods Sixty female SD rats were randomly divided into sham operation group,blank group,model group,electroacupuncture group,and medicine group,with 12 rats in each group.The rats in blank group received no treatment.The bilateral ovaries of rats in the sham operation group were only exposed but not removed,while bilateral ovarian resection was performed in the other groups to establish the osteoporosis model.After 13 weeks of modeling,rats in the electroacupuncture group were stimulated at Shenshu(BL23),Pishu(BL20),Sanyinjiao(SP6),and Zusanli(ST36)points for 15 min(intermittent wave,2 Hz,1.0mA)and were fed with the same volume of distilled water to the other groups.The treatment was once a day and two days off after lasting 5 days.Rats in the medicine group received estradiol 0.02 mg/mL,1 mL/100 g weight by gavage,and electroacupuncture.Rats in the rest groups were fed with an equal volume of distilled water,and the electroacupuncture was the same.The rats in each group were weighed once a week and then the dosage of medicine was adjusted.The rats were killed under anesthesia after the experiment.The femurs and the tibia were isolated.The gene and protein expression of wnt3a and β-catenin of the femurs and the tibia was detected using RT-PCR analysis and immunohistochemistry.Results The expression of Wnt3a and β-catenin protein in model group was significantly lower than that in the blank group and Sham operation group(P<0.05).The expression of Wnt3a and β-catenin protein increased significantly after acupuncture or medicine intervention compared with the model group,with statistical significance(P<0.05).The mRNA expression of Wnt3a and β-catenin was the lowest in the model group,and it significantly decreased(P<0.05)compared with that in the blank group and Sham operation group.After acupuncture or medicine intervention,the mRNA expression of Wnt3a and β-catenin significantly elevated,which was statistically significant compared with the model group(P<0.05).Conclusion The mechanism of electroacupuncture in the treatment of postmenopausal osteoporosis may be through regulating the gene and protein expression of wnt3a and β-catenin,and activating the Wnt/β-catenin signaling pathway,then regulating the balance of bone remodeling.