文献类型：期刊文献

中文题名：miR-143-3p在胃癌组织中的表达和临床意义研究以及生信分析

英文题名：The expression and clinical significance of miR-143-3p in gastric cancer tissues with bioinformatical analysis

作者：牛荣[1];王转兄[2];白悦[1];赵文涛[1];呼永华[2];田一虹[2];李海龙[2]

第一作者：牛荣

机构：[1]甘肃省肿瘤医院胸外一科;[2]甘肃中医药大学临床医学院

第一机构：甘肃省肿瘤医院胸外一科

年份：2019

卷号：26

期号：5

起止页码：538

中文期刊名：中国普外基础与临床杂志

外文期刊名：Chinese Journal of Bases and Clinics in General Surgery

收录：CSTPCD

基金：甘肃省高等学校科研项目(项目编号:甘财教[2014]63-15);甘肃省高等学校科研项目计划(项目编号:2016A-045)

语种：中文

中文关键词：胃癌;miR-143-3p;肿瘤分子标志物;生信分析

外文关键词：gastric cancer;miR-143-3p;tumor molecular marker;bioinformatics analysis

摘要：目的探索miR-143-3p在胃癌细胞和胃癌组织中的表达、并分析其异常表达的临床意义;同时采用生物信息学方法,进行靶基因和信号通路富集分析。方法采用实时荧光定量PCR法检测miR-143-3p在正常胃黏膜细胞和5种胃癌细胞,以及胃癌组织和癌旁组织中的表达,并分析其临床意义。采用OncomiR和YM500数据库分析miR-143-3p在胃癌组织和癌旁组织中的表达。采用miRecords网站数据库的预测软件预测其靶基因,选择至少3个以上软件支持的靶基因,运用DAVID 6.7软件在线富集分析其靶基因参与的信号通路。结果实验结果表明:相对于正常胃黏膜上皮细胞GES-1,miR-143-3p在不同分化程度的胃癌细胞,包括SGC-7901(中分化)、MKN-45(中分化)、MGC-803(低分化)、BGC-823(低分化)和HGC-27(未分化)细胞中的表达量均明显下调(P<0.001)。实时荧光定量PCR检测结果表明:相对于癌旁组织,miR-143-3p在胃癌组织中的表达在36例中下调,在18例中上调,4例变化不明显。miR-143-3p在胃癌组织中的表达与胃癌淋巴结转移以及浸润深度相关(P<0.05)。生信分析结果显示,miR-143-3p的靶基因富集在38个与肿瘤相关的信号通路中。结论 miR-143-3p是胃癌中表达下调的分子标志物,是潜在的具有临床相关性的抑癌基因,可能参与胃癌发生发展过程中的生物学行为。
Objective To observe the expressions of miR-143-3 p in gastric cancer cells and gastric carcinoma tissues with its clinical significance, and to analyze the target genes with enriched pathway by using bioinformatics methods. Methods The expressions of miR-143-3 p in different differentiation gastric cancer cells and normal gastric mucosa cell line, and the expressions in gastric cancer tissues and adjacent tissues were detected by real-time fluorescent quantitative PCR. In addition, OncomiR and YM500 databases were used to analyze the expression of miR-143-3 p in gastric cancer tissues compared with adjacent tissues. Furthermore, the targets of miR-143-3 p were predicted by using the software of miRecords website database, and at least three software-supported target genes were chosen to analyze the enriched the signal pathways in which the target gene was involved with DAVID 6.7 software. Results The expressions of miR-143-3 p in the different differentiation degree of gastric cancer cells compared with normal gastric mucosa cell line were downregulated(P<0.001), and the expression of miR-143-3 p in gastric cancer tissues compared with adjacent tissues was also downregulated(downregulated in 36 cases, upregulated in 18 cases, and no alteration in 4 cases). The expression of miR-143-3 p in gastric cancer tissues was associated with lymph node metastasis and invasion depth(P<0.05).Bioinformatics analysis results showed that the target genes of miR-143-3 p were enriched in 38 signaling pathways associated with cancer. Conclusion MiR-143-3 p is a down-regulated molecular marker in gastric cancer and a potentially clinically related tumor suppressor gene, which may be involved in the cancerous phenotype in carcinogenesis and development of gastric cancer.