文献类型：期刊文献

英文题名：Carbon ion irradiation conquers the radioresistance by inducing complex DNA damage and apoptosis in U251 human glioblastomas cells

作者：Guo, Yulu[1,2];Li, Pingping[1,2];Zhang, Jinhua[1,2];Hao, Sijia[1,2];Zhou, Xuan[4];Di, Cuixia[1,2,3];Long, Feng[4];Zhang, Hong[1,2,3];Si, Jing[1,2,3]

第一作者：Guo, Yulu

通信作者：Zhang, H[1];Si, J[1];Zhang, H[2];Si, J[2];Zhang, H[3];Si, J[3]

机构：[1]Chinese Acad Sci, Inst Modern Phys, Dept Med Phys, 509 Nanchang Rd, Lanzhou 730000, Gansu, Peoples R China;[2]Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China;[3]Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou, Peoples R China;[4]Gansu Univ Chinese Med, Sch Basic Med, Lanzhou, Peoples R China

第一机构：Chinese Acad Sci, Inst Modern Phys, Dept Med Phys, 509 Nanchang Rd, Lanzhou 730000, Gansu, Peoples R China

通信机构：[1]corresponding author), Chinese Acad Sci, Inst Modern Phys, Dept Med Phys, 509 Nanchang Rd, Lanzhou 730000, Gansu, Peoples R China;[2]corresponding author), Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China;[3]corresponding author), Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou, Peoples R China.

年份：2025

卷号：42

期号：3

外文期刊名：MEDICAL ONCOLOGY

收录：;Scopus(收录号：2-s2.0-85217993864);WOS:【SCI-EXPANDED(收录号:WOS:001412886100001)】；

基金：This study was financially supported by the National Key R and D Program of China (2018YFE0205100), Scientific Technology Research Projects of Gansu Province (24JRRA031), Lanzhou Talent Innovation and Entrepreneurship Project (2021-RC-82), the Science and Technology Plan Project of Chengguan district (2019RCCX0071), and Guangdong Basic and Applied Basic Research Foundation (2021A1515010027).

语种：英文

外文关键词：Radioresistance; Carbon ions; DNA damage response; Apoptosis

摘要：Glioblastoma multiforme (GBM) is the most malignant brain tumor, with radiotherapy frequently employed following surgical resection. However, conventional radiation therapies often yield suboptimal results. This study investigated the effects of X-ray and carbon ion irradiation on the glioblastoma cell line U251 to assess the distinctive advantages of carbon ion treatment and explore mechanisms for overcoming radiation resistance. The findings indicated that carbon ion irradiation more effectively inhibited colony formation and induced more severe apoptosis and cell cycle disorder in U251 cells. Immunofluorescence assays revealed larger and more abundant Upsilon-H2AX and 53BP1 foci in the carbon ion irradiation group. Western blot analysis demonstrated that carbon ion-induced DNA damage repair involved a complex array of pathways, with the RAD51-mediated homologous recombination (HR) pathway being predominant, while the Rad23B-mediated nucleotide excision repair (NER) pathway and XRCC1-mediated base excision repair (BER) were more relevant in response to X-ray irradiation. These results suggest that carbon ion irradiation may overcome radioresistance by inducing more complex DNA damage and apoptosis, thus providing insights for targeting new strategies in combining gene therapy with radiotherapy.