文献类型：期刊文献

英文题名：Xuan-Fei-Hua-Zhuo Decoction Against LPS-Induced Acute Lung Injury by Regulating the Permeability and Microtubule Stabilization of Pulmonary Microvascular Endothelial Cells in Mice

作者：Zhu, Peng[1];Cui, Nan[2];Feng, Dapeng[3];Li, Yumin[4];Lv, Qin[1];Cao, Zhijun[1];Wang, Fengjiao[1];Li, Zhiguo[1];Xu, Qian[5];Zhang, Lumei[5];Hao, Guoxing[5];Liu, Yu[5];Zhang, Zhiming[6];Xu, Xin[1]

第一作者：Zhu, Peng

通信作者：Xu, X[1];Zhang, ZM[2]

机构：[1]Beijing Fengtai Hosp Integrated Tradit Chinese & M, 60 Sanlijia, Beijing 100072, Peoples R China;[2]Chinese Acad Tradit Chinese Med, Xiyuan Hosp, Beijing, Peoples R China;[3]Gansu Prov Cent Hosp, Lanzhou, Peoples R China;[4]Gansu Second Peoples Hosp, Lanzhou, Peoples R China;[5]Gansu Univ Tradit Chinese Med, Lanzhou, Peoples R China;[6]Gansu Prov Hosp Tradit Chinese Med, 418 Guazhou Rd, Lanzhou 730050, Gansu, Peoples R China

第一机构：Beijing Fengtai Hosp Integrated Tradit Chinese & M, 60 Sanlijia, Beijing 100072, Peoples R China

通信机构：[1]corresponding author), Beijing Fengtai Hosp Integrated Tradit Chinese & M, 60 Sanlijia, Beijing 100072, Peoples R China;[2]corresponding author), Gansu Prov Hosp Tradit Chinese Med, 418 Guazhou Rd, Lanzhou 730050, Gansu, Peoples R China.

年份：2025

卷号：267

期号：4

起止页码：419

外文期刊名：TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE

收录：;Scopus(收录号：2-s2.0-105025945991);WOS:【SCI-EXPANDED(收录号:WOS:001655291500005)】；

基金：Transformation in Gansu Province (zyzx-2020-03) ; Research Project of National Administration of Traditional Chinese Medicine (2021ZYLCYJ08) ; Major Special Project of Science and Technology Plan of Gansu Province (22ZD1FA001) ; Lanzhou Talent Innovation and Entrepreneurship Project (2021-RC-80) ; Research Project of Hebei Provincial Administration of Traditional Chinese Medicine (B2025007) ; Special Program for Training Newly Employed Young Researchers of the Chinese Academy of Traditional Chinese Medicine (ZZ17-XRZ-003) ; Beijing Fengtai Hospital of Integrated Traditional and Western Medicine Natural Science Foundation (YS2022-01) ; Research Project of Gansu Provincial Administration of Traditional Chinese Medicine (GZKP-2023-23) and Major Science and Technology Project-Leading Talents in Science and Technology (21ZDKA0014) .r Transformation in Gansu Province (zyzx-2020-03) ; Research Project of National Administration of Traditional Chinese Medicine (2021ZYLCYJ08) ; Major Special Project of Science and Technology Plan of Gansu Province (22ZD1FA001) ; Lanzhou Talent Innovation and Entrepreneurship Project (2021-RC-80) ; Research Project of Hebei Provincial Administration of Traditional Chinese Medicine (B2025007) ; Special Program for Training Newly Employed Young Researchers of the Chinese Academy of Traditional Chinese Medicine (ZZ17-XRZ-003) ; Beijing Fengtai Hospital of Integrated Traditional and Western Medicine Natural Science Foundation (YS2022-01) ; Research Project of Gansu Provincial Administration of Traditional Chinese Medicine (GZKP-2023-23) and Major Science and Technology Project-Leading Talents in Science and Technology (21ZDKA0014) .

语种：英文

外文关键词：acute lung injury; lung vascular permeability; microtubule stabilization; traditional Chinese medicine

摘要：Acute lung injury (ALI) is an acute bilateral pulmonary infiltration disease, which may finally cause impairment and even loss of lung function. This study aimed to investigate the therapeutic potential and the mechanism of the Xuanfei Huazhuo decoction (XFHZ) against ALI in mice. The ALI mice stimulated by lipopolysaccharide (LPS) were subjected to the treatment of saline, 0.06, 0.11, and 0.22 mg/kg of XFHZ, and 10 mg/kg of fasudil, respectively, for seven consecutive days. It was found that XFHZ significantly attenuated LPS-induced pathological injury and mitochondrial dysfunction of vascular endothelial cells in the lung and suppressed LPS-mediated lung pulmonary edema (lung wet/dry weight ratio), the elevation of vascular permeability (increased total protein and albumin content in bronchoalveolar lavage fluid) and neutrophil infiltration. Microtubule stabilization, a process that could be regulated by GEF-H1, MYPT-1, Tau, and MAP-4, is critical for maintaining the endothelial cell barrier, of which disruption is a pathological hallmark of ALI. XFHZ reduced the expression of GEF-H1 and MYPT-1 at mRNA and protein levels and decreased Tau and MAP-4 protein expression in LPS-induced ALI. XFHZ also suppressed the increase of monomeric tubulin and the decrease of polymeric tubulin in injured lung induced by LPS. This study demonstrated that XFHZ can improve LPS-induced ALI by promoting microtubule stabilization, providing a theoretical basis for the clinical treatment of patients with ALI induced by different factors, including SARS-CoV-2 infection.