文献类型：期刊文献

中文题名：甘草不同药用部位甘草酸及甘草苷含量比较研究

英文题名：Comparative study on glycyrrhizic acid and liquiritin contents in different medicinal parts of Glycyrrhizae Radix et Rhizoma

作者：程芳[1,2];吴志成[1];韩静[1];贺沙沙[1]

第一作者：程芳

机构：[1]甘肃中医药大学附属医院制剂中心,甘肃兰州730020;[2]敦煌医学与转化教育部重点实验室,甘肃兰州730000

第一机构：甘肃中医药大学第二附属医院

年份：2024

卷号：41

期号：1

起止页码：57

中文期刊名：甘肃中医药大学学报

外文期刊名：Journal of Gansu University of Chinese Medicine

基金：甘肃省中医药科研项目(GZK-2013-10);敦煌医学与转化教育部重点实验室开放基金项目(DHYX18-20)。

语种：中文

中文关键词：甘草主根;甘草尾;甘草稍;甘草酸;甘草苷;高效液相色谱法;含量测定

外文关键词：Glycyrrhizae Radix et Rhizoma taproot;modified subterraneous stem;fibrous root;glycyrrhizic acid;liquiritin;HPLC;content determination

摘要：目的比较甘草主根、甘草稍、甘草尾中甘草酸及甘草苷含量。方法采用高效液相色谱法测定甘草主根、甘草稍、甘草尾中2个指标性成分甘草酸、甘草苷的含量。色谱柱为Thermo Hypersil Gold-C_(18)(4.6 mm×250.0 mm,5μm),流动相为乙腈-0.5%磷酸水,梯度洗脱,流速1.0 mL/min,检测波长甘草酸为254 nm、甘草苷为275 nm,柱温30℃,进样量10μL。结果甘草苷和甘草酸的回归方程分别为Y=2×10^(-6)X-13905(r=0.9999),Y=699804X-9419(r=0.9999),线性范围分别为0.172~2.064,0.218~2.616μg;平均加样回收率分别为96.50%,96.06%,RSD分别为1.70%,1.03%;甘草主根中2种指标性成分的平均含量最高,甘草稍次之,甘草尾相对最低。结论甘草主根、甘草稍、甘草尾的质量存在差异,为保证中医临床用药的有效性,应合理选择甘草药用部位。
Objective To compare the contents of glycyrrhizic acid and liquiritin in taproot,modified subterraneous stem and fibrous root of Glycyrrhizae Radix et Rhizoma.Methods HPLC was used to determine the contents of two index components(glycyrrhizic acid,liquiritin)in taproot,modified subterraneous stem and fibrous root of Glycyrrhizae Radix et Rhizoma.The determination was performed on Thermo Hypersil Gold-C_(18)(4.6 mm×250.0 mm,5μm)column with mobile phase consisting of acetonitrile-0.5%phosphate water at the flow rate of 1.0 mL/min.The detection wavelength was 254 nm for glycyrrhizic acid and 275 nm for liquiritin.The column temperature was 30℃.The sample size was 10μL.Results The regression equations of liquiritin and glycyrrhizic acid were Y=2×10^(-6)X-13905(r=0.9999),Y=699804X-9419(r=0.9999),and the linear ranges were 0.172~2.064 and 0.218~2.616μg,respectively.The average sample recovery rate was 96.50%,96.06%,RSD was 1.70%,1.03%;the average content of 2 index components in taproots was the highest,followed by modified subterraneous stems,and the lowest in fibrous roots.Conclusion The contents of glycyrrhizic acid and liquiritin in taproot,modified subterraneous stem and fibrous root of Glycyrrhizae Radix et Rhizoma are different.In order to ensure the effectiveness of clinical medication,the medicinal part should be selected reasonably.