文献类型：期刊文献

中文题名：黄芪多糖联合顺铂对小鼠Lewis肺癌移植瘤Caspase-3、Smac/Diablo表达的影响

英文题名：Astragalus polysaccharide inhibiting Lewis lung carcinoma transplanted tumor in mice and influences the expression of Caspase-3 and Smac/Diablo

作者：庄梦婕[1,2];刘丹[1,2];陈彦文[3,2];明海霞[4,2]

第一作者：庄梦婕

机构：[1]甘肃中医药大学;[2]甘肃省中药药理与毒理学重点实验室甘肃省高校重大疾病分子医学与中医药防治研究重点实验室,兰州730000;[3]甘肃中医药大学基础医学院;[4]甘肃中医药大学临床医学院

第一机构：甘肃中医药大学

年份：2018

卷号：49

期号：1

起止页码：63

中文期刊名：解剖学报

外文期刊名：Acta Anatomica Sinica

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：甘肃省高校基本科研业务项目(2014);甘肃省中医药管理局项目(GZK-2017-6);兰州市科技局计划项目(2014-1-23);甘肃中医药大学研究生创新基金(2017CX31)

语种：中文

中文关键词：黄芪多糖;顺铂;Lewis肺癌;免疫组织化学;免疫印迹法;小鼠

外文关键词：Astragalus polysaccharides;Cisplatin;Lewis lung carcinoma;Immunohistochemistry;Western blotting;Mouse

摘要：目的观察黄芪多糖(APS)及联合顺铂(DDP)对小鼠Lewis肺癌(LLC)移植瘤凋亡蛋白Caspase-3、Smac/Diablo表达的影响,探讨黄芪多糖抗肿瘤的机制。方法 90只C57BL/6 J小鼠,随机分为9组,正常组、模型组、APS低、中、高剂量组、DDP组、联合低、中、高剂量组,每组10只。除正常组外,其余80只均接种肺癌移植瘤细胞(1×1010/L)于右前肢腋窝皮下,制造模型为荷瘤小鼠。制造模型次日起,治疗组的小鼠给予腹腔注射0.3 ml药物。顺铂每周注射1次,其余药物每日1次,正常组和模型组注射等体积生理盐水,连续20 d,于第21天处死。肿瘤组织进行HE染色并行病理学观察;免疫组织化学染色和图像分析方法检测移植瘤细胞中的Caspase-3及Smac/Diablo的表达。结果荷瘤小鼠在APS(高)、联合(中)、联合(高)组的体质量变化,具有统计学意义。与模型组小鼠比较,肿瘤组织的病理组织学HE显示,APS(高)联合顺铂组的肿瘤细胞坏死最为明显;免疫组织化学法表明,治疗组的肿瘤组织中Caspase-3、Smac/Diablo蛋白表达水平均升高,联合(高)组升高最明显。结论 APS及联合化疗药物DDP能抑制小鼠Lewis肺癌细胞的生长,其机制可能与升高Caspase-3、Smac/Diablo的表达有关。
Objective To observe the effect of Astragalus polysaccharides（ APS） combined with cisplatin（ DDP） on the expressions of Caspase-3 and Smac/Diablo in the mice with transplantated tumors Lewis lung carcinoma（ LLC）. Methods Ninety C57 BL/6 J mice were randomly divided into normal control group,model group,and 50,100 or 200 mg/L APS group,6 mg/kg cisplatin group,and 3 mg/kg cisplatin combined with 50,100 or 200 mg/L APS group;10 mice per group. Except the mice in normal group,the rest mice were inoculated with LLC cells（ 1 × 1010/L） in the right fore axillary subcutaneous tissue to establish a model of tumor-bearing mice. In the second day of building the animal model,the mice in the treatment group were given intraperitoneal injection of 0. 3 ml of the drug. At the same time,the mice in the cisplatin group were given once a week,and the rest of the group mice once a day. The mice in the normal and model groups were given the same amount of saline injection for 20 days. All mice were killed on the 21 st day. Tumor tissue lesions were observed by HE staining. The expression and location of Caspase-3 and Smac/Diablo proteins in transplanted tumor tissues were detected by immunohistochemical staining and image analysis method. Results Theweights of mice were decreased in the 100 and 200 mg/L APS group and 3 mg/kg cisplatin combined with 50,100,200 mg/L APS group. Compared with the model group,the necrosis of tumor tissues in the 200 mg/L APS combined with 3 mg/kg cisplatin group was most obvious. The expression of Caspase-3 and Smac/Diablo was increased in the treatment group. The increasing of tumor tissues in 200 mg/L APS combined with 3 mg/kg cisplatin group was most obvious.Conclusion APS and APS combined with cisplatin restrain the growth of Lewis lung cancer in C57 BL/6 J mouse,which may depend on increase of the expression of Caspase-3 and Smac/Diablo.