文献类型：期刊文献

中文题名：奇应内消巴布剂定性定量方法研究

英文题名：Study on the quality and quantity methods of Qiyingneixiao cataplasm*

作者：余琰[1];魏舒畅[1];韩涛[1];金辉[1];范凌云[1]

第一作者：余琰

机构：[1]甘肃中医学院

第一机构：甘肃中医药大学

年份：2012

卷号：32

期号：1

起止页码：132

中文期刊名：药物分析杂志

外文期刊名：Chinese Journal of Pharmaceutical Analysis

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD2011_2012】；

基金：甘肃省科技攻关项目(2GS064-A43-020-24)

语种：中文

中文关键词：奇应内消巴布剂;薄层色谱;高效液相色谱;重楼;大黄;山柰;大黄酸;大黄素;大黄酚;橡胶膏剂;中药质量标准

外文关键词：Qiyingneixiao cataplasm ; TLC ; HPLC ; Parids Rhizoma; Rhei Radix et Rhizoma ; Kaempferiae Rhizoma ; rhein ; emodin ; chrysophanol ; rubber ointment ; TCM quality standard

摘要：目的:建立奇应内消巴布剂定性定量方法。方法:采用TLC法鉴别方中重楼、大黄、山柰;采用HPLC法测定大黄酸、大黄素、大黄酚含量。HPLC色谱条件:采用Agilent ZORBAX SB-C18(4.6 mm×250 mm,5μm)色谱柱,流动相为甲醇-0.2%磷酸水溶液(87∶13),流速1.0 mL.min-1,检测波长440 nm,柱温30℃。结果:定性鉴别薄层色谱特征明显;HPLC测定,大黄酸浓度在4.00～25.00μg.mL-1范围内线性关系良好(r=0.9994),平均回收率(n=5)为98.39%(RSD=2.0%);大黄素浓度在17.92～112.00μg.mL-1范围内线性关系良好(r=0.9998),平均回收率(n=5)为97.25%(RSD=1.5%);大黄酚浓度在33.92～212.00μg.mL-1范围内线性关系良好(r=0.9992),平均回收率(n=5)为96.55%(RSD=1.8%)。结论:该法可以准确地对奇应内消巴布剂进行定性、定量检测,有效地控制该制剂的质量。
Objective： To establish the quality and quantity methods of Qiyingneixiao cataplasm. Methods： Parids Rhizoma, Rhei Radix et Rhizoma, Kaempferiae Rhizoma in this preparation were identified by TLC. The content of rhein ,emodin and chrysophanol in Qiyingneixiao cataplasm were determined by HPLC. HPLC method was performed on a Agilent Zorbax SB Cl8 column（4. 6 mm ×250 mm,5 μm） with a mobile phase of methanol -0. 2% phosphoric acid. The wavelength of detector was set at 440 nm and the column temperature was 30 ℃. Results： Characteristics of identification were very obviously. The linear range of rhein was from 4.00 to 25.00 μg · mL ^- 1 （ r =0. 9994） and the average recovery（n = 5）was 98. 39% （RSD = 2.0% ）;The linear range of emodin was from 17.92 to 112. 00 μg · mL ^- 1 （ r = 0. 9998 ） and the average recovery （ n = 5 ） was 97.25 % （ RSD = 1.5 % ） ; The linear range of ehrysophanol was from 33.92 to 212.00 μg · mL ^- 1 （r =0. 9992） and the average recovery（ n =5 ）was 96.55% （RSD = 1.8% ）. Conclusion： The methods of qualitative and quantitative analysis are accurate and suitable for effectively controlling quality of Qiyingneixiao cataplasm.