文献类型：期刊文献

中文题名：党参超微粉碎工艺优选及溶出度考察

英文题名：Optimization of Ultrafine Pulverization Technology of Codonopsis Radix and Investigation of Its Dissolution

作者：窦霞[1,2];靳子明[2];狄留庆[1]

第一作者：窦霞

机构：[1]南京中医药大学;[2]甘肃中医学院附属医院

第一机构：南京中医药大学

年份：2014

卷号：20

期号：9

起止页码：23

中文期刊名：中国实验方剂学杂志

外文期刊名：Chinese Journal of Experimental Traditional Medical Formulae

收录：CSTPCD;;北大核心:【北大核心2011】；

基金：甘肃省中医药管理局科研项目(GZK-2011-83)

语种：中文

中文关键词：党参;超微粉碎工艺;正交试验;溶出度试验

外文关键词：Codonopsis Radix; ultrafine pulverization process; orthogonal test; dissolution test

摘要：目的:优选党参的超微粉碎工艺并考察其体外溶出度。方法:以党参炔苷提取量为指标,通过正交试验考察加入药粉量、原粉末粒度、粉碎时间对党参超微粉碎工艺的影响;采用HPLC测定党参炔苷含量,流动相乙腈-水(20∶80),检测波长268 nm;利用桨状搅拌法测定溶出度,比较超微粉和普通粉不同时间溶出液中党参炔苷含量。结果:党参最佳超微粉碎工艺为加入药粉量1 000 g,原粉末粒度65目,粉碎时间40 min;党参炔苷平均提取量3.95 mg·g-1,RSD 2.85%。超微粉溶出液比普通粉溶出液中党参炔苷含量明显提高,党参超微粉在30 min时溶出率已达80%,而普通粉末120 min时溶出率仅有40%。结论:优选的超微粉碎工艺稳定可行,党参超微粉中党参炔苷的溶出速率远大于党参普通粉。
Objective：To optimize ultrafine pulverization process of Codonopsis Radix and investigate its in vitro dissolution.Method：Taking extraction amount of lobetyolin as index,orthogonal test was adopted to optimize ultrafine pulverization process with powder consumption,preliminary powder fineness and pulverization time as factors.HPLC was employed to determine the content of lobetyolin with mobile phase of acetonitrile-water （20∶ 80） and detection wavelength of 268 nm.Dissolution was determined by Paddle-shaped mixing method,the content of lobetyolin in stripping liquid at different times from uhrafine powder and ordinary powder was compared.Result：Optimal ultrafine pulverization technology was as follows：preliminary powder fineness 65 mesh,powder consumption 1 000 g,pulverization time 40 minutes; Average extraction amount of lobetyolin was 3.95 mg ·g^-1 with RSD of 2.85%.Compared with ordinary powder,the content of lobetyolin in stripping liquid from ultrafine powder increased by 35%,dissolution of ultrafine powder in 30 min was up to 80%,while it was 40% in 120 min for ordinary powder.Conclusion：This optimized technology was stable and feasible,dissolution rate of lobetyolin in ultrafine powder was much larger than ordinary powder.