文献类型：期刊文献

英文题名：A potential marker of radiation based on 16S rDNA in the rat model: Intestinal flora

作者：Zhang, Liying[1];Miao, Zhiming[1];Li, Yangyang[1];Xu, Xiaomin[1,2];Zhou, Ting[1];Zhang, Yiming[1];Liu, Yongqi[1,3]

第一作者：张利英

通信作者：Liu, YQ[1];Liu, YQ[2]

机构：[1]Gansu Univ Chinese Med, Prov Level Key Lab Mol Med Major Dis & Prevent &, Lanzhou, Gansu, Peoples R China;[2]Hexi Univ, Med Coll, Zhangye, Gansu, Peoples R China;[3]Gansu Univ Chinese Med, Key Lab Dunhuang Med & Transformat Prov & Minister, Lanzhou, Gansu, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Prov Level Key Lab Mol Med Major Dis & Prevent &, Lanzhou, Gansu, Peoples R China;[2]corresponding author), Gansu Univ Chinese Med, Key Lab Dunhuang Med & Transformat Prov & Minister, Lanzhou, Gansu, Peoples R China.|[10735]甘肃中医药大学;

年份：2023

卷号：18

期号：8

外文期刊名：PLOS ONE

收录：;Scopus(收录号：2-s2.0-85166111653);WOS:【SCI-EXPANDED(收录号:WOS:001041953300016)】；

基金：We confirm that the following projects have funded us National Natural Science Foundation of China, 82260882 Li-Ying Zhang National Natural Science Foundation of China, 82004094 YongQi Liu Longyuan Youth Innovation and Entrepreneurship Talent Project in 2021, GZTZ[2021]17-1 Li-Ying Zhang Natural Science Foundation of Gansu Province, 20JR10RA318 Li-Ying Zhang Natural Science Foundation of Gansu Province, 20JR10RA332 YongQi Liu China Postdoctoral Science Foundation Project, 2021M693794 Li-Ying Zhang Lanzhou City Health Key Science and Technology Development Project, 2021006 Li-Ying Zhang Funders YongQi Liu and Li-Ying Zhang conceived and designed the experiments, and provided the funding required for the experiments.

语种：英文

摘要：The gastrointestinal microbiota plays an important role in the function of the host intestine. However, little is currently known about the effects of irradiation on the microorganisms colonizing the mucosal surfaces of the gastrointestinal tract. The aim of this study was to investigate the effects of X-ray irradiation on the compositions of the large intestinal Microbiotas of the rat. The gut microbiotas in control mice and mice receiving irradiation with different dose treatment were characterized by high-throughput sequencing of the bacterial 16S rDNA gene and their metabolites were detected by gas chromatography-mass spectrometry. Unexpectedly, the diversity was increased mildly at 2Gy irradiation, and dose dependent decreased at 4Gy, 6Gy, 8Gy irradiation. The phyla with large changes in phylum level are Firmicutes, Bacteroides and Proteobacteria; the abundance ratio of Firmicutes/Bacteroides is inverted; and when 8Gy is irradiated, the phylum abundance level was significantly increased. At the genus level, the abundance levels of Phascolarctobacterium, Ruminococcaceae and Lachnospiraceae increased at 2Gy irradiation, and significantly decreased at 4Gy, 6Gy, and 8Gy irradiation; the abundance level of Prevotellaceae diminished at 2Gy irradiation, and enhanced at 4Gy, 6Gy, 8Gy irradiation; The abundance level of Violet bacteria (Christenellaceae) and Lactobacillus attenuated in a dose-dependent manner; Lachnoclostridium enhanced in a dose-dependent manner; Bacteroides was in 4Gy, 6Gy, 8Gy The abundance level increased significantly during irradiation; the abundance level of Shigella (Escherichia-Shigella) only increased significantly during 8Gy irradiation. Lefse predicts that the biomarker at 0Gy group is Veillonellaceae, the biomarker at 2Gy group is Firmicutes, the biomarkers at 4Gy group are Dehalobacterium and Dehalobacteriaceae, the biomarkers at 6Gy group are Odoribacter, and the biomarkers at 8Gy group are Anaerotruncus, Holdemania, Proteus, Bilophila, Desufovibrionales and Deltaproteobacteria. Overall, the data presented here reveal that X-ray irradiation can cause imbalance of the intestinal flora in rats; different doses of irradiation can cause different types of bacteria change. Representative bacteria can be selected as biomarkers for radiation damage and repair.This may contribute to the development of radiation resistance in the future.