文献类型：期刊文献

中文题名：痛泻要方对溃疡性结肠炎大鼠结肠组织病理形态及血清中相关因子含量的影响

英文题名：Influence of Tongxieyao Formula on Inflammatory Factors in Rats with Ulcerative Colitis

作者：朱向东[1];李兰珍[1];段永强[1];曹燕飞[1];汪斌[1]

第一作者：朱向东

机构：[1]甘肃中医学院,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2013

卷号：20

期号：4

起止页码：41

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2013_2014】；

基金：国家自然科学基金(81060283)

语种：中文

中文关键词：痛泻要方;溃疡性结肠炎;白细胞介素-1β;肿瘤坏死因子-α;白细胞介素-4;大鼠

外文关键词：Tongxieyao Formula; ulcerative colitis; IL-1β; TNF-α; IL-4; rats

摘要：目的观察痛泻要方对溃疡性结肠炎(UC)大鼠结肠组织病理形态及血清中相关因子的影响,探讨其作用机制。方法 60只SPF级Wistar大鼠随机分为空白组、模型组、柳氮磺胺吡啶(SASP)组及痛泻要方低、中、高剂量组,除空白组外,其余各组大鼠均以2,4,6-三硝基苯磺酸(TNBS)/乙醇灌肠法制备UC大鼠模型,同时,痛泻要方低、中、高剂量组分别按原药材11、22、44 g/kg剂量灌胃,SASP组按0.3 g/kg剂量灌胃,灌胃体积均为10 mL/kg,空白组、模型组灌服等体积生理盐水,持续3周。HE染色观察大鼠结肠组织病理形态并评分,采用ELISA法检测大鼠血清白细胞介素(IL)-1β、肿瘤坏死因子(TNF-α)和IL-4水平。结果 HE染色显示,各治疗组结肠组织病理形态明显改善;与空白组比较,模型组大鼠血清IL-1β、TNF-α含量均明显升高,IL-4含量显著下降(P<0.05,P<0.01);与模型组比较,痛泻要方高、中、低剂量组大鼠血清IL-1β、TNF-α水平降低,而IL-4水平明显升高(P<0.05,P<0.01)。结论痛泻要方通过下调促炎因子IL-lβ、TNF-α的表达,升高抗炎因子IL-4的表达,调节肠道异常免疫反应、抑制炎症反应,修复溃疡,进而防治UC。
Objective To observe the curative effect and mechanism of Tongxieyao Formula on ulcerative colitis（UC）.Methods Sixty SPF Wistar rats were randomly divided into blank group,model group,SASP group and low-,medium-,high-dose of Tongxieyao Formula groups.Except the blank group,UC models were made by means of TNBS/ethanol enema.The low-,medium-,high-dose of Tongxieyao Formula groups were given Tongxieyao Formula 11,22,44 g/kg by gavage,SASP group was given 0.3 g/kg,the volume was 10 mL/kg.The blank group and model group were given with the same volume of normal physiological saline for three weeks.The pathological morphology of colon was observed and scored by hematoxylin-eosin staining（HE）,IL-1β,TNF-α and IL-4 in rat serum were detected by ELISA.Results Compared with the model group,the colon tissue pathological morphology was significantly improved in all treatment groups.Compared with the blank group,the content of IL-1β and TNF-α in the model group was increased,and the content of IL-4 was decreased significantly（P 0.05,P 0.01）.The content of IL-1β and TNF-α in high-,medium-,low-dose of Tongxieyao Formula groups were lower than that in the model group,and the content of IL-4 was higher（P 0.05,P 0.01）.Conclusion Tongxieyao Formula can decrease the expression of lL-lβ and TNF-α,increase the expression of IL-4,regulate immune abnormalities of intestine,inhibit inflammation,repair ulcers,and then prevent and treat UC.