文献类型：期刊文献

中文题名：不同剂量骨碎补对兔骨髓间充质干细胞增殖分化的影响

英文题名：Drynaria freeze-dried powder at different dosages influences proliferation and differentiation of rabbit bone marrow mesenchymal stem cells

作者：张虎林[1];张自强[2];许宏斌[1];张晓刚[1]

第一作者：张虎林

机构：[1]甘肃中医学院附属医院;[2]宝鸡市中医院

第一机构：甘肃中医药大学第二附属医院

年份：2014

卷号：18

期号：41

起止页码：6649

中文期刊名：中国组织工程研究

外文期刊名：Chinese Journal of Tissue Engineering Research

收录：CSTPCD;;CSCD:【CSCD_E2013_2014】；

基金：甘肃省科技支撑计划-社会发展计划项目(0804NKCA101)~~

语种：中文

中文关键词：干细胞;骨髓干细胞;骨碎补;骨髓间充质干细胞;增殖;诱导分化;组织工程学;兔

外文关键词：bone marrow;mesenchymal stem cells;polypodiaceae;cellproliferation

摘要：背景:现代研究表明骨碎补具有推迟细胞退行性变、降低骨关节病发病率的作用。目的:比较不同剂量骨碎补冻干粉诱导兔骨髓间充质干细胞增殖、分化的效果,并筛选出最佳剂量。方法:利用密度梯度离心法结合贴壁筛选法分离培养兔骨髓间充质干细胞,分为空白组、转化生长因子β1阳性对照组、骨碎补高、中、低剂量组(分别含骨碎补0.4 mg,0.1 mg,5μg),取第3代骨髓间充质干细胞,根据实验分组加入不同培养液进行培养,1周后MTT比色法检测活细胞数目,免疫组化法测定Ⅱ型胶原表达。结果与结论:MTT比色结果显示转化生长因子β1和骨碎补冻干粉均能促进骨髓间充质干细胞增殖,转化生长因子β1阳性对照组>骨碎补低剂量组>骨碎补中剂量组>骨碎补高剂量组>空白组。细胞免疫组化检测Ⅱ型胶原结果显示转化生长因子β1和骨碎补冻干粉均可以诱导兔骨髓间充质干细胞向软骨细胞分化,诱导后的细胞明显表达Ⅱ型胶原,转化生长因子β1阳性对照组>骨碎补低剂量组>骨碎补中剂量组>骨碎补高剂量组>空白组。结果表明骨碎补能促进兔骨髓间充质干细胞增殖和分化,尤以5μg剂量效果最佳。
BACKGROUND：Modern research shows that Drynaria can delay celldegeneration and reduce the incidence of osteoarthritis. OBJECTIVE：To observe the proliferation and differentiation of rabbit bone marrow mesenchymal stem cells induced by different dosages of Drynaria freeze-dried powder, and to explore the optimum induction concentration. METHODS：Rabbit bone marrow mesenchymal stem cells were isolated and cultured in vitro by density gradient centrifugation and adherence screening methods, and then divided into blank group, positive control group （transforming growth factor β1）, high-, middle-, low-dosage Drynaria groups （0.4 mg, 0.1 mg, 5μg）. Passage 3 cells were selected and cultured in different media. After 1 week, cellviability was detected by MTT method, and expression of type II col agen by immunohistochemical method. RESULTS AND CONCLUSION：Both transforming growth factorβ1 and Drynaria could improve the proliferation of bone marrow mesenchymal stem cells, and the increase in cellproliferation was ranked as fol ows：positive control group〉low-dosage group〉middle-dosage group〉high-dosage group〉blank group. Bone marrow mesenchymal stem cells were differentiated into chondrocytes under induction of transforming growth factorβ1 and Drynaria, and induced cells significantly expressed type II col agen. The expression of type II col agen was ranked as fol ows：positive control group〉low-dosage group〉middle-dosage group〉high-dosage group〉blank group. These findings suggest that Drynaria can promote the proliferation and differentiation of rabbit bone marrow mesenchymal stem cells, and the optimal dosage is 5μg.