文献类型：期刊文献

中文题名：病证结合大鼠辐射损伤模型构建初探

英文题名：Preliminary study on the establishment of rat model of radiation damage with combination of disease and syndrome

作者：梁建庆[1,2];李金田[1,2];李娟[1];刘永琦[1];蔺兴遥[1];安耀荣[1];刘凤艳[3];张子同[1];李晓伟[1]

第一作者：梁建庆

机构：[1]甘肃中医药大学敦煌医学与转化省部共建教育部重点实验室;[2]甘肃中医药大学博士后流动站;[3]甘肃中医药大学附属医院

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2017

卷号：0

期号：5

起止页码：2142

中文期刊名：中华中医药杂志

外文期刊名：China Journal of Traditional Chinese Medicine and Pharmacy

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；

基金：国家自然科学基金地区项目(No.81460695);中国博士后科学基金第60批面上项目(No.2016 M602887)~~

语种：中文

中文关键词：病证结合;动物模型;辐射损伤;大鼠

外文关键词：Combination of disease and syndrome;;Animal model;;Radiation damage;;Rat

摘要：目的:建立一种稳定的、可操作性强的病证结合大鼠辐射损伤模型,以满足研究药物对辐射损伤防治评价的需求。方法:采用碳离子(^(12)C^(6+))束照射大鼠右侧肺部构建辐射损伤动物模型,同时设立正常对照组、单纯辐射组、辐射+黄芪汤组,从病因、症状、客观指标、药物反证等方面对辐射损伤大鼠模型进行综合判断,验证该模型的中医证候属性。结果:单纯辐射组大鼠的症状体征(摄食量、饮水量、心率、肛温、排尿量、体质量)、血象(WBC与RBC)、脾脏、胸腺指数等指标与正常对照组比较,差异均有统计学意义(P<0.01)。辐射+黄芪汤组可明显改善大鼠的主观症状和上述客观指标,差异有统计学意义(P<0.01)。结论:^(12)C^(6+)束照射大鼠辐射损伤模型的中医证候属性是气阴两虚证。
Objective: To establish a stable and simple operation rat model of radiation damage with combination of disease and syndrome to meet the demand of study the medication on preventionand evaluationof radiation damage. Methods: The animal model of radiation damagewas constructed by using ^(12)C^(6+) beam irradiation on the right lung of rats.At the same time, the normal control group, single radiation group and radiation + Huangqi Decoction group were set up. The rat model of radiation damage was estimatedfrom the etiology, symptom, objective index and drug counter evidence, in order to investigate the syndrome property of this rat model. Results: Compared with the normal control group, the symptom and sign, hemogram and organ index in single radiation group were significantly different(P<0.01). The radiation +Huangqi Decoction group could significantly improve the subjective symptom and the above objective indexes, and the difference was statistically significant(P<0.01). Conclusion: The TCM syndrome of rat modelof radiation damage constructed by ^(12)C^(6+) beam irradiation is syndrome of deficiency of both qi and yin.