文献类型：期刊文献

英文题名：Effects of PGPR consortium and amino acids on rhizosphere microbial communities and plant performance in Lanzhou lily

作者：Meng, Lingyu[1,2];Li, Xianshu[1,2];Sun, Erqin[1,2];Ma, Xiaohui[1,2];Wang, Yinquan[1,2,3];Jin, Ling[1,2,3]

第一作者：Meng, Lingyu

通信作者：Wang, YQ[1];Jin, L[1]

机构：[1]Gansu Univ Chinese Med, Coll Pharm, Lanzhou, Peoples R China;[2]Gansu Pharmaceut Ind Innovat Res Inst, Lanzhou, Peoples R China;[3]Northwest Collaborat Innovat Ctr Tradit Chinese Me, Beijing, Peoples R China

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Coll Pharm, Lanzhou, Peoples R China.|[1073501e14fb35863569f]甘肃中医药大学药学院（西北中藏药协同创新中心办公室）;[10735]甘肃中医药大学;

年份：2025

外文期刊名：PLANT AND SOIL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001571182600001)】；

基金：We thank Shanghai OE Biotech Co., Ltd. and Luming Biology Co., Ltd. for their assistance in data support.

语种：英文

外文关键词：Lanzhou lily; PGPR consortium; Amino acids; Rhizosphere microbiome; Bulb metabolomics

摘要：AimsContinuous cropping of Lanzhou lily disrupts rhizosphere microecosystem balance, severely compromising bulb quality. This study aims to explore the effect of plant growth-promoting rhizobacteria (PGPR) consortium with amino acids (AAs) on continuous cropping obstacles and to elucidate the regulatory mechanisms of rhizosphere microbiota and bulb metabolism.MethodsA three-year field experiment compared three treatments: control (CK), PGPR consortium (T1), and PGPR consortium with AAs (T2). The study evaluated soil enzymes and physicochemical factors, bulb yield and quality index, microbial community, and metabolite spectrum.ResultsThe PGPR consortium improved agronomic performance and rhizosphere function, and the effect is further enhanced after adding AAs. Compared to CK, T1 and T2 significantly increased bulb yield by 20.78% And 28.29%, respectively. Both treatments enhanced bulb polysaccharides, proteins, and saponins, and reduced soil pH and organic matter (OM) content. T1 and T2 promoted beneficial bacteria (TRA3-20, Ellin6055, and Gaiella), and suppressed pathogenic fungi (Cadophora). T2 enriched keystone taxa (MND1 and Nitrospira) while reducing pathogens (Acremonium and Ilyonectria). Metabolomics revealed that T1 and T2 altered cyanoamino acid metabolism, increasing stress-responsive metabolites (L-valine and L-isoleucine) and enhanced active substances such as L-ascorbic acid and trigonelline. T2 also elevated organic acids and modulated C5-branched dibasic acid metabolism, facilitating sugar accumulation.ConclusionThe integration of PGPR consortium with AAs markedly influenced the restructuring of the rhizosphere microbiome and bulb metabolic activity. This study elucidates the interactions between keystone microbial taxa and host metabolic processes, laying the groundwork for novel strategies to address continuous cropping challenges in Lanzhou lily.