文献类型：期刊文献

中文题名：AMI-1通过下调PRMT5表达对胰腺癌细胞体外活性的影响

英文题名：Effects of AMI-1 on the activity of pancreatic cancer cells in vitro by down-regulating PRMT5 expression

作者：张景惠[1,2];卫浩亮[1,2];王丽[1,2];李京凯[1,2];张宝来[3];杨孝来[1,2,3]

第一作者：张景惠

机构：[1]甘肃中医药大学药学院药理教研室,甘肃兰州730000;[2]甘肃省人民医院药剂科,甘肃兰州730000;[3]兰州大学药理研究所,甘肃兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2023

卷号：28

期号：6

起止页码：601

中文期刊名：中国临床药理学与治疗学

外文期刊名：Chinese Journal of Clinical Pharmacology and Therapeutics

收录：CSTPCD;;CSCD:【CSCD2023_2024】；

基金：国家自然科学基金地区项目(81560595)。

语种：中文

中文关键词：AMI-1;胰腺癌;PRMT5;体外活性

外文关键词：AMI-1;pancreatic cancer;PRMT5;in vitro activity

摘要：目的:研究AMI-1对人胰腺癌MIAPaca-2细胞增殖、迁移、凋亡的影响及其机制。方法:以MIAPaca-2细胞为研究对象,分别设立对照组,不同浓度AMI-1(0.6、1.2、2.4 mmol/L)处理组。采用MTT、克隆形成实验、Transwell和划痕实验分别检测AMI-1对MIAPaca-2细胞增殖、克隆、迁移的影响;流式细胞术检测细胞凋亡;Western blot检测AMI-1对MIAPaca-2细胞中caspase3、cleaved-caspase3、PRMT5、H4R3me2s、PCNA蛋白表达的影响。结果:与对照组相比,不同浓度AMI-1处理后,MIAPaca-2细胞的存活率逐渐降低,呈浓度和时间依赖性(P<0.01);细胞克隆形成率减少(P<0.01),细胞迁移能力减弱(P<0.01),细胞凋亡率升高(P<0.01),cleaved-caspase3/caspase3蛋白表达升高(P<0.01),PRMT5、H4R3me2s和PCNA的蛋白表达降低(P<0.01)。结论:AMI-1能够抑制胰腺癌细胞体外生长增殖、迁移和诱导凋亡,可能与AMI-1下调PRMT5、H4R3me2s和PCNA的表达,上调cleaved-caspase3/caspase3的表达有关。
AIM:To explore the effect and mechanism of AMI-1 on the proliferation,migration and apoptosis of human pancreatic cancer MIAPaca-2 cells.METHODS:MIAPaca-2 cells were randomly divided into the control groups and the treatment groups with different concentrations of AMI-1(0.6,1.2 and 2.4 mmol/L).MTT assay,colony formation assay,scratch assay and Transwell assay respectively were used to detect the effects of AMI-1 on the proliferation,colony formation and migration of MIAPaca-2 cells.The cell apoptosis was detected by flow cytometry.The effect of AMI-1 on the protein expression of caspase3,cleaved-caspase3,PRMT5,H4R3me2s and PCNA in MIAPaca-2 cells were detected by western blot.RESULTS:Compared with control group,after treatment with different concentrations of AMI-1,the cell survival rate of MIAPaca-2 cells decreased gradually in a time-and dose-dependent manner(P<0.01),the rate of cell clone formation decreased(P<0.01),the cell migration ability were diminished(P<0.01),the apoptosis rate was increased(P<0.01),and the protein level of cleaved-caspase3/caspase3 were increased,the protein level of PRMT5,H4R3me2s and PCNA were reduced(P<0.01).CONCLUSION:AMI-1 significantly inhibited the proliferation migration and apoptosis of pancreatic cancer cells in vitro,which may be associated with AMI-1 down-regulating the expression of PRMT5,H4R3me2s and PCNA,and up-regulation the expression of cleaved-caspase3/caspase3.