文献类型：期刊文献

中文题名：当归多糖对炎性微环境中骨髓间充质干细胞增殖及STAT3信号通路的影响

英文题名：Effects of angelica sinensis polysaccharide on BMSCs proliferation and STAT3 signaling pathway in inflammatory microenvironment

作者：任春贞[1];安涛[2];赵信科[1];刘永琦[3];骆亚莉[3]

第一作者：任春贞

机构：[1]甘肃中医药大学附属医院,兰州730000;[2]国家心血管病中心,北京协和医学院,中国医学科学院阜外医院,北京100010;[3]甘肃中医药大学,兰州730000

第一机构：甘肃中医药大学第二附属医院

年份：2020

卷号：35

期号：10

起止页码：5274

中文期刊名：中华中医药杂志

外文期刊名：China Journal of Traditional Chinese Medicine and Pharmacy

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD_E2019_2020】；

基金：国家自然科学基金项目(No.81860786)。

语种：中文

中文关键词：炎性微环境;骨髓间充质干细胞;当归多糖;信号转导和转录激活因子-3信号通路;细胞增殖;生物学特性;白介素-6;转化生长因子-β1

外文关键词：Inflammatory microenvironment;Bone marrow mesenchymal stem cells;Angelica sinensis polysaccharides;STAT3 signaling pathway;Proliferation;Biological characteristic;IL-6;TGF-β1

摘要：目的:探讨当归多糖(ASP)对炎性微环境中骨髓间充质干细胞(BMSCs)增殖及信号转导和转录激活因子-3(STAT3)信号通路的影响。方法:采用CCK-8法分别筛选白介素-6(IL-6)和转化生长因子-β1(TGF-β1)促进BMSCs细胞增殖的最佳浓度;以最佳浓度的IL-6和TGF-β1建立炎性微环境,同时加入ASP干预微环境,实验分为6组:BMSCs组、TGF-β1组、IL-6组、IL-6+TGF-β1组、ASP+IL-6组和ASP+IL-6+TGF-β1组;46d后,通过微丝染色和CCK-8法分别检测各组细胞骨架及增殖的变化,Western Blot技术检测炎性微环境中各组细胞STAT3信号通路相关蛋白的表达变化。结果:50ng/mL IL-6和2ng/mL TGF-β1具有明显促进BMSCs细胞增殖的作用,并且高浓度IL-6和TGF-β1并不能加强对BMSCs细胞的促增殖作用;与BMSCs组比较,IL-6组、IL-6+TGF-β1组细胞增殖显著加快(P<0.05),STAT3及P-STAT3蛋白表达均显著升高(P<0.05);与IL-6组比较,ASP+IL-6组细胞增殖减慢(P<0.05),STAT3及P-STAT3蛋白表达均下调(P<0.05);与IL-6+TGF-β1组比较,ASP+IL-6+TGF-β1组细胞增殖减慢(P<0.05),STAT3及P-STAT3蛋白表达均下调(P<0.05)。结论:炎性微环境中BMSCs细胞生物学特性发生改变,该变化可能与IL-6和TGF-β1可导致BMSCs中STAT3信号通路激活有一定相关性;ASP可抑制BMSCs在炎性微环境中生物学特性的变化。
Objective:To investigate the effects of angelica sinensis polysaccharide(ASP)on the proliferation of BMSCs and STAT3 signaling pathway in inflammatory microenvironment.Methods:The optimal concentrations of IL-6 and TGF-β1 to promote the proliferation of BMSCs cells were screened by CCK-8 method;the inflammatory microenvironment was established with the optimal concentrations of IL-6 and TGF-β1,and ASP was added to interfere with the microenvironment.Divided into 6 groups:BMSCs group,TGF-β1 group,IL-6 group,IL-6+TGF-β1 group,ASP+IL-6 group and ASP+IL-6+TGF-β1 group;46 days later,microfilament staining and CCK-8 were used to detect changes in cytoskeleton and proliferation in each group.Western Blot was used to detect the expression of STAT3 signaling pathway-related proteins in each group of cells in the inflammatory microenvironment.Results:IL-6 at 50 ng/mL and TGF-β1 at 2 ng/mL could obviously promote the proliferation of BMSCs cells,and high concentrations of IL-6 and TGF-β1 could not strengthen the effect on BMSCs.Compared with the BMSCs group,the proliferation of cells in the IL-6 group and the IL-6+TGF-β1 group was significantly accelerated(P<0.05),and the expression of STAT3 and P-STAT3 proteins were significantly increased(P<0.05).Compared with the IL-6 group,cell proliferation in the ASP+IL-6 group was slowed down(P<0.05),and STAT3 and P-STAT3 protein expressions were down-regulated(P<0.05).Compared with the IL-6+TGF-β1 group,cell proliferation was slowed in the ASP+IL-6+TGF-β1 group(P<0.05),and STAT3 and P-STAT3 protein expression was down-regulated(P<0.05).Conclusion:The biological characteristics of BMSCs cells change in the inflammatory microenvironment.This change may be related to the activation of the STAT3 signaling pathway in BMSCs by inflammatory factors.ASP can inhibit the changes of the biological characteristics of BMSCs in the inflammatory microenvironment.