文献类型：期刊文献

英文题名：Heavy ion beams and gamma rays induce biological changes and flavonoid accumulation in Astragalus mongholicus Bunge

作者：Wang, Yuanmeng[1,2];Li, Xuehu[2,3];Liu, Xiao[2];Ge, Linghui[1,2];Kang, Guisen[2,3];Du, Yan[2,3];Wang, Fusheng[4];Chen, Fanglei[5];Li, Ping[2,3];Jin, Ling[1,2,6];Zhou, Libin[1,2,3]

第一作者：王亚梅;王苑铭;Wang, Yuanmeng

通信作者：Jin, L[1];Zhou, LB[1];Jin, L[2];Zhou, LB[2];Zhou, LB[3];Jin, L[4]

机构：[1]Gansu Univ Chinese Med, Coll Pharm, Lanzhou, Peoples R China;[2]Chinese Acad Sci, Inst Modern Phys, Biophys Grp, Lanzhou, Peoples R China;[3]Univ Chinese Acad Sci, Beijing, Peoples R China;[4]Dingxi Acad Agr Sci, Dingxi, Peoples R China;[5]State Nucl Secur Technol Ctr, Beijing, Peoples R China;[6]Northwest Collaborat Innovat Ctr Tradit Chinese Me, Lanzhou, Peoples R China

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Coll Pharm, Lanzhou, Peoples R China;[2]corresponding author), Chinese Acad Sci, Inst Modern Phys, Biophys Grp, Lanzhou, Peoples R China;[3]corresponding author), Univ Chinese Acad Sci, Beijing, Peoples R China;[4]corresponding author), Northwest Collaborat Innovat Ctr Tradit Chinese Me, Lanzhou, Peoples R China.|[1073501e14fb35863569f]甘肃中医药大学药学院（西北中藏药协同创新中心办公室）;[10735]甘肃中医药大学;

年份：2024

卷号：334

外文期刊名：SCIENTIA HORTICULTURAE

收录：;Scopus(收录号：2-s2.0-85193828831);WOS:【SCI-EXPANDED(收录号:WOS:001245933100001)】；

基金：This work was supported by grants from Crop Varietal Improvement and Insect Pests Control by Nuclear Radiation; Natural Science Foun-dation of Gansu Province (22JR5RA119) ; National Key Research and Development Program (2022YFD1200705) ; Ecological Planting and Quality Assurance Project of Daodi Medicinal Materials (China Science and Technology for Traditional Chinese Medicine (2020) 153) ; China Agriculture Research System of MOF and MARA (CARS-21) ; Strategic Scientia Horticulturae 334 (2024) 113327 Research and Consulting Project of Chinese Academy of Engineering (GS2021ZDA06) ; Dingxi Municipal Science and Technology Program (DX2021AN10) .

语种：英文

外文关键词：Astragalus mongholicus Bunge; Heavy ion beams; Gamma rays; Biological effects; Flavonoids

摘要：Astragalus mongholicus Bunge (A. mongholicus) is a perennial herb in the Fabaceae family. Its dried rhizome has been used in clinical practice in China for more than 2000 years. However, the limited germplasm resources and unstable quality of A. mongholicus have limited its large-scale cultivation and wide application. Therefore, there is an urgent need to breed stable varieties. At present, heavy ion beams (HIBs) and gamma rays are important mutagenesis tools applied in medicinal plants. The aim of this investigation was to assess the effects of various doses (0, 50, 100, 200, and 400 Gy) of HIBs and gamma rays on the development, physiological reaction, and secondary metabolite accumulation of A. mongholicus. The findings indicated that the LD50 of the A. mongholicus seeds to HIBs and gamma rays was 200-250 Gy and 400 Gy, respectively. As the radiation dose increased, there was a subsequent increase in the inhibition of seedling height, plant diameter, and number of branches and leaves, as well as a decrease in the chlorophyll a, b, and carotenoid contents of leaves. Additionally, the chlorophyll fluorescence parameter NPQ and flavonoid contents (total flavonoids, Calycosin-7-glucoside, Calycosin, Ononin, and Methylnissolin-3-O-glucoside) were also affected, which resulted in a distinct decrease in Y(II) values and diverse levels of elevation in leaf MDA content, SOD, and POD activities. Differently, CAT activity was substantially reduced, except for 50 Gy HIBs irradiation. Overall, upon the same dose of the two radiation qualities, HIBs had a stronger inhibitory effect on the growth of Astragalus seedlings, but less effect on antioxidant enzymes and physiological responses. There was no difference between two radiation qualities in increasing trend of flavonoid composition. The present study showed that HIBs is more beneficial for A. membranaceus mutation breeding research and would be promising tool for A. mongholicus mutation breeding.