文献类型：期刊文献

英文题名：A novel biosensor based on boronic acid functionalized metal-organic frameworks for the determination of hydrogen peroxide released from living cells

作者：Dai, Hongxia[1,2,4];Lu, Wenjuan[1,2];Zuo, Xianwei[1,2];Zhu, Qian[1,2];Pan, Congjie[1,2];Niu, Xiaoying[1,2];Liu, Juanjuan[1,2];Chen, HongLi[1,2];Chen, Xingguo[1,2,3]

第一作者：Dai, Hongxia;戴红霞

通信作者：Lü, WJ[1];Chen, XG[1]

机构：[1]Lanzhou Univ, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China;[2]Lanzhou Univ, Dept Chem, Lanzhou 730000, Peoples R China;[3]Key Lab Nonferrous Met Chem & Resources Utilizat, Lanzhou 730000, Peoples R China;[4]Gansu Univ Chinese Med, Lanzhou 730000, Peoples R China

第一机构：Lanzhou Univ, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China.

年份：2017

卷号：95

起止页码：131

外文期刊名：BIOSENSORS & BIOELECTRONICS

收录：;EI(收录号：20171703596168);Scopus(收录号：2-s2.0-85018508670);WOS:【SCI-EXPANDED(收录号:WOS:000402219700020)】；

基金：The authors are grateful for financial support from the National Natural Science Foundation of China (No. 21375053) and the Specialized Research Fund for the Doctoral Program of Higher Education (No. 20130211110039).

语种：英文

外文关键词：Metal-organic frameworks; Boronic acid; Hydrogen peroxide; Horseradish peroxidase; Electrochemical biosensor

摘要：In this work, we report a durable and sensitive 11202 biosensor based on boronic acid functionalized metal organic frameworks (denoted as MIL-100(Cr)-B) as an efficient immobilization matrix of horseradish peroxidase (HRP). MIL-100(Cr)-B features a hierarchical porous structure, extremely high surface area, and sufficient recognition sites, which can significantly increase HRP loading and prevent them from leakage and deactivation. The H2O2 biosensor can be easily achieved without any complex processing. Meanwhile, the immobilized HRP exhibited enhanced stability and remarkable catalytic activity towards H2O2 reduction. Under optimal conditions, the biosensor showed a fast response time (less than 4 s) to H2O2 in a wide linear range of 0.5-3000 mu M with a low detection limit of 0.1 mu M, as well as good anti-interference ability and long-term storage stability. These excellent performances substantially enable the proposed biosensor to be used for the real-time detection of H2O2 released from living cells with satisfactory results, thus showing the potential application in the study of H2O2-involved dynamic pathological and physiological process.