文献类型：期刊文献

中文题名：芍药苷对溃疡性结肠炎模型大鼠结肠组织病理形态及血清抗炎因子和促炎因子平衡的影响

英文题名：Influence of Paeoniflorin on Contents of Interleukin - 1β,Interleukin - 4 and TNF - α in Rats with Ulcerative Colitis

作者：朱向东[1];曹燕飞[1];王燕[1];段永强[1];荀敏齐[1];汪斌[1]

第一作者：朱向东

机构：[1]甘肃中医学院基础课部

第一机构：甘肃中医药大学

年份：2013

卷号：40

期号：9

起止页码：1927

中文期刊名：辽宁中医杂志

外文期刊名：Liaoning Journal of Traditional Chinese Medicine

收录：CSTPCD

基金：国家自然科学基金地区基金(81060283)

语种：中文

中文关键词：芍药苷;溃疡性结肠炎;IL-1β;TNF-α;IL-4

外文关键词：Paeoniflorin ; ulcerative colitis ; IL - 1 β ; TNF - α; IL - 4

摘要：目的:观察芍药苷对溃疡性结肠炎(UC)大鼠白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、白细胞介素4(IL-4)的影响。方法:将实验动物分为4组,采用TNBS/乙醇灌肠法造模,光镜观察结肠组织形态并评分,采用ELISA法检测大鼠血清IL-1β、TNF-α、IL-4水平。结果:模型组大鼠结肠组织黏膜层可见溃疡形成,且有明显的炎症、充血存在,证实造模成功。模型组大鼠血清IL-1β、TNF-α水平均高于空白组(P<0.05),而血清IL-4水平则低于空白组(P<0.01);治疗后,芍药苷组血清IL-1β、TNF-α水平均较模型组降低(P<0.05、P<0.01),而血清IL-4水平则较模型组升高(P<0.01)。结论:芍药苷能够通过下调IL-1β、TNF-α和升高IL-4的表达,调节肠道异常免疫反应、抑制炎症反应,修复溃疡,进而治疗UC。
Objective ： To observe the influence of the Paeoniflorin on Interleukin - 1β （ IL - 1 β ） , TNF - αt （ Tumor Necrosis Factor-α ）and Interleukin-4 （IL-4）in rats with ulcerative colitis（ UC ）. Methods：The experimental animals were divided into four groups to prepare the rat models by means of the TNBS/ethanol enema, observing the colon organization form under the light microscope and then giving the score ; the levels of IL - 1β, TNF - αand IL - 4 in rat serum were detected by enzyme linked im-munosorbent assay（ELISA）. Results：Colon mucosa of the model group showed ulceration ,and had obvious inflammation and con-gestion, which meant the modeling succeeded. The contents of IL - 1β and TNF - α in rats in the model group were transparently higher than those in the blank control group（ P 〈 0. 05 ）, and the content of IL - 4 was lower than it（ P 〈 0. 01 ） ; after treatment, compared with the model group,the Paeoniflorin group＇s contents of IL - 1β and TNF -α were reduced（P 〈0.05 ,P 〈0. 01 ）, and the content of IL- 4 was increased（ P 〈 0. 01 ）. Conclusion：The Paeoniflorin can reduce the expressions of IL-1 15 and TNF or,increase the expression of IL - 4, regulate immune abnormalities in intestines, inhibit inflammation, repair ulcers, and cure UC.