文献类型：期刊文献

中文题名：红芪总黄酮对高糖致脐静脉内皮细胞损伤保护作用的研究

英文题名：Protective Effect of Total Flavonoids of Hedysarum Polybotry on Human Umbilical Vein Endothelial Cells Injury Treated by High Glucose

作者：陈彻[1];楚惠媛[1];杨雅丽[1];王雅莉[1];董建勇[2]

第一作者：陈彻

机构：[1]甘肃中医学院科研中心,甘肃兰州730000;[2]温州医学院药学院,浙江温州325035

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2007

卷号：19

期号：2

起止页码：84

中文期刊名：甘肃科学学报

外文期刊名：Journal of Gansu Sciences

收录：CSTPCD

基金：甘肃省自然科学中青年基金项目(NOYS031-A21-015)

语种：中文

中文关键词：红芪总黄酮;脐静脉内皮细胞;高糖;抗氧化

外文关键词：Total flavonoids of Hedysarum polybotry; umbilical vein endothelial cells; high glucose

摘要：研究了红芪总黄酮对高糖处理的脐静脉内皮细胞损伤的保护作用.使用MTT法检测细胞存活率.化学比色法检测乳酸脱氢酶(LDH)、丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性.结果表明:红芪总黄酮可明显改善高糖所致下降的细胞生存率,显著降低LDH释放及细胞内MDA含量,提高SOD活性.红芪总黄酮对高糖处理的脐静脉内皮细胞损伤具有保护作用,其作用机制可能与提高脐静脉内皮细胞的抗氧化能力有关.
To investigate protective effect of total flavonoids of Hedysarum polybotry on human umbilical vein endothelial cells injury treated by high glucose. The growth rate of cells was detected by MTT （3-（4, 5-dimethylthiazo 1-2-yl）-2, 5-diphenylte trazolium bromide） methods. Lactate dehydrogenase （LDH） in the culture medium, malondialdelyde （MDA） and superoxide dismutase （SOD） in the cell were measured by spectroscopy, respectively. Result： Total flavonoids of Hedysarum polybotrys could significantly improve the growth of cells which is injured by high glucose, and decrease the content of LDH, MDA and increase SOD activity. Conclusion： Total flavonoids of Hedysarum polybotry has protective effect on human umbilical vein endothelial cells injury treated by high glucose, which may be related to increasing the antioxidant capacity of human umbilical vein endothelial cells.