文献类型：期刊文献

中文题名：西黄丸对模型大鼠抗乳腺增生作用及其机制研究

英文题名：Effect and its mechanism of Xihuang Pill on hyperplasia of mammary glands in rats

作者：连小龙[1];赵敏[1];韩涛[1];韩宇[2];王秋兰[3]

第一作者：连小龙

机构：[1]甘肃中医药大学药学院,兰州730000;[2]华中科技大学生命科学与技术学院,武汉430074;[3]甘肃中医药大学临床医学院,兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2017

卷号：33

期号：23

起止页码：2390

中文期刊名：中国临床药理学杂志

外文期刊名：The Chinese Journal of Clinical Pharmacology

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；

基金：国家自然科学基金资助项目(81560722)

语种：中文

中文关键词：西黄丸;乳腺增生;激素水平;雌二醇;孕酮

外文关键词：Xihuang Pill ; hyperplasia of mammary gland ; hormone level; estradiol ; progesterone

摘要：目的研究西黄丸对乳腺增生大鼠血清激素水平及乳腺组织病理变化影响。方法按照体重将Wistar大鼠随机分为6组:正常组、模型组、对照组(他莫昔芬片1.8mg·kg^(-1)·d^(-1))和低、中、高3个低剂量实验组(西黄丸0.54,1.08,2.16 g·kg^(-1)·d^(-1))。除正常组外,其余5组动物均建立乳腺增生模型,建模的同时,均灌胃给药,正常组和模型组灌胃等体积的蒸馏水。以酶联免疫吸附法检测血清中雌二醇(E2)、孕酮、泌乳素(PRL)和睾酮水平,用立体定量学方法观察乳腺小叶体密度、间质体密度和腺泡比表面。结果正常组、模型组、对照组、低、中、高3个剂量实验组的E2分别为(44.16±10.63),(65.92±26.98),(47.56±10.95),(50.05±11.84),(50.06±14.30),(48.42±12.48)pg·m L-1;这6组的孕酮分别为(38.26±12.42),(23.24±6.03),(36.77±4.75),(33.11±13.26),(33.04±12.07),(37.75±7.45)ng·m L-1;这6组的PRL分别为(113.23±77.83),(253.06±104.9),(159.58±83.25)(168.96±68.76),(130.28±84.44)(142.77±90.36)ng·m L^(-1);这6组的睾酮分别为(4.01±0.67),(5.46±1.08),(4.24±1.72),(4.69±1.07),(4.64±0.54),(4.29±0.98)ng·m L-1,与正常组比较,模型组差异均有统计学意义(均P<0.01);与模型组比较,实验组差异均有统计学意义(P<0.05,P<0.01)。正常组、模型组、对照组、低、中、高3个剂量实验组的乳腺小叶体密度分别为(37.82±6.37)%,(93.25±5.63)%,(40.12±8.31)%,(70.33±7.56)%,(65.58±7.31)%,(50.33±5.24)%;这6组的小叶间质体密度分别为(62.18±6.37)%,(6.75±5.63)%,(59.88±8.31)%,(29.67±7.56)%,(34.42±7.31)%,(49.67±5.24)%;这6组的腺泡比表面分别为(0.92±0.19),(2.31±0.23),(1.11±0.18)%,(1.93±0.26)%,(1.54±0.57)%,(1.20±0.30)%,与正常组比较,模型组差异均有统计学意义(均P<0.01);与模型组比较,实验组差异均有统计学意义(P<0.05,P<0.01)。结论西黄丸能够抑制雌二醇和黄体酮所致的大鼠乳腺增生。
Objective To study the anti-hyperplasia of mammary glands effect of Xihuang Pill and its mechanisms in rats. Methods Sixty Wistar rats were equally divided into 6 groups： normal group,model group,control group and low,middle,high dose experimental groups.Except the normal group,the other 5 groups were established the animal models of mammary gland hyperplasia, while modeling, the rats in control group was treated with the dose of 1. 8 mg·kg^（-1）·d-1 tamoxifen,the rats in experimental groups were treated with the dose of 0. 54,1. 08,2. 16 g·kg^（-1）·d-1 Xihuang Pill; the rats in the normal group and the model group were fed with equal volume of distilled water. The serumlevels of estradiol（ E2）,progesterone（ P）,prolactin（ PRL）,testosterone（ T） were detected by enzyme league immune assay. The lobular breast density,lobular interstitial density,and alveolar surface area were observed by three-dimensional quantitative method. Results The levels of E2 in normal group,model group,control group,experimental-L group,experimental-M group and experimental-H groups were（ 44. 16 ± 10. 63）,（ 65. 92 ± 26. 98）,（ 47. 56 ± 10. 95）,（ 50. 05 ± 11. 84）,（ 50. 06 ± 14. 30）,（ 48. 42 ± 12. 48） pg·m L-1; the levels of P in the six groups were（ 38. 26 ± 12. 42）,（ 23. 24 ± 6. 03）,（ 36. 77 ± 4. 75）,（ 33. 11 ± 13. 26）,（ 33. 04 ± 12. 07）,（ 37. 75 ± 7. 45）ng·m L-1; the levels of PRL in the six groups were（ 113. 23 ± 77. 83）,（ 253. 06 ± 104. 90）,（ 159. 58 ± 83. 25）,（ 168. 96 ± 68. 76）,（ 130. 28 ± 84. 44）,（ 142. 77 ± 90. 36） ng · m L-1,the levels of T in the six groups were（ 4. 01 ± 0. 67）,（ 5. 46 ± 1. 08）,（ 4. 24 ± 1. 72）,（ 4. 69 ± 1. 07）,（ 4. 64 ± 0. 54）,（ 4. 29 ± 0. 98） ng·m L-1. Compared with normal group,the differences in model group were statistically significant（ all P〈0. 01）. Compared with model group,the difference in experimental groups was statistically significant（ P〈0. 05,P〈0. 01）. Lobular breast density in normal group,model group,control group,experimental-L group,experimental-M group and experimental-H groups were（ 37. 82 ± 6. 37） %,（ 93. 25 ± 5. 63） %,（ 40. 12 ± 8. 31） %,（ 70. 33 ± 7. 56） %,（ 65. 58 ± 7. 31） %,（ 50. 33 ±5. 24） %; Lobular interstitial density in the six groups were（ 62. 18 ± 6. 37） %,（ 6. 75 ± 5. 63） %,（ 59. 88 ±8. 31） %,（ 29. 67 ±7. 56） %,（ 34. 42 ±7. 31） %,（ 49. 67 ±5. 24） %. Alveolarsurface areain the six groups were（ 0. 92 ±0. 19） %,（ 2. 31 ±0. 23） %,（ 1. 11 ±0. 18） %,（ 1. 93 ±0. 26） %,（ 1. 54 ± 0. 57） %,（ 1. 20 ± 0. 30） %. Compared with normal group,the difference in model group was statistically significant（ all P〈0. 01）. Compared with model group,the difference in experimental groups was statistically significant（ P〈0. 05,P〈0. 01）. Conclusion Xihuang Pill can inhibit the hyperplasia of mammary glands induced by estradiol and progesterone in rats.